紫外和圆二色光谱研究三环唑与牛血清白蛋白的相互作用  

The Interaction between Tricyclazole and Bovine Serum Albumin by UV and Circular Dichroism

作　　者：丁成荣[1] 高晓茹[1] 许莉[2] 张国富[1] 周瑛[1] 

机构地区：[1]浙江工业大学化学工程与材料学院,杭州310032 [2]浙江大学化学系,杭州310027

出　　处：《农药》2010年第1期29-30,38,共3页Agrochemicals

基　　金：浙江工业大学应用化学省重中之重学科开放基金资助(56310101622)

摘　　要：通过紫外(UV)和圆二色(CD)光谱,研究三环唑与牛血清白蛋白(BSA)的相互作用及其浓度变化对牛血清白蛋白(BSA)的影响。研究表明:三环唑对牛血清白蛋白的影响是多方面的。紫外吸收光谱表明三环唑与牛血清白蛋白(BSA)借助分子间力形成超分子基态复合物,此外由于它们之间的疏水作用,导致BSA紫外吸收强度随三环唑浓度增大逐渐增强;CD光谱表明三环唑与BSA间的疏水作用使BSA的肽链发生收缩、重排。三环唑导致BSA的构象发生变化。The interaction between tricyclazole and bovine serum albumin （BSA） and the effect of different tricyclazole concentration to bovine serum albumin （BSA） had been studied by ultraviolet （UV） and circular dichroism （CD） spectroscopy, The results showed that the effect of tricyclazole to bovine serum albumin was multifaceted. The absorption spectra of UV showed that tricyclazole and bovine serum albumin （BSA） form the ground-state supramolecutar complex with intermolecular forces. At the same time, hydrophobic interactions lead to a gradual increase intensity of UV absorption. The CD spectra showed that the peptide chain of BSA occurs contraction and rearrangement because of hydrophobic interactions and intramolecular hydrogen bonds. The conformation of BSA had been changed.

关 键 词：紫外光谱 圆二色谱 牛血清白蛋白 三环唑 

分 类 号：S482.2[农业科学—农药学]