葡萄砧木‘F-242’组织培养及快繁技术  

Establishment of Tissue Culture and Rapid Propagation System of Grape Rootstock ‘F-242’

作　　者：刘伟[1] 李希东[1] 刘新[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109

出　　处：《北方园艺》2010年第1期68-70,共3页Northern Horticulture

基　　金：农业部948资助项目(2006-G26)

摘　　要：以葡萄砧木品种‘F-242’带芽新梢为供试材料,研究了消毒时间、激素种类及其浓度配比、基本培养基对外植体生根的影响。结果表明:75%乙醇15 s,0.1%的升汞8 min为葡萄砧木品种‘F-242’最佳的外植体消毒时间。附加生长素类物质IAA和IBA可以明显促进‘F-242’外植体的生根,且IAA处理的综合效果要明显优于IBAI、AA的最佳附加浓度为0.1 mg/L。不同的基本培养基对葡萄品种‘F-242’外植体生根影响显著,其中以MS基本培养基为最佳。In order to establish the efficient rapid propagation system of grape rootstock ＇F-242＇,the effects of different disinfection times, hormones and their concentration ratio, basic mediums on rooting of explants were studied. The results indicated that the influence of different disinfection times were significantly different. The explants were sanitized in 75% alcohol for 15 s and then treated in 0.1% mercuric chloride for about 8 min,contamination can be controlled efficiently. The treatment which adding IAA and IBA could promote rooting of exptants efficiently and the comprehensive effect of adding IAA was better then IBA. 0. 1 mg/L was the best adding concentration of IAA. The different basic mediums on rooting of explants were significantly different and MS was the best.

关 键 词：葡萄 'F-242’外植体 生根率 

分 类 号：S663.104.3[农业科学—果树学]