牦牛催乳素受体基因部分片段的克隆与序列分析  

Cloning and sequence analysis of partial fragment of prolactin receptor gene in yaks

作　　者：陈达文[1] 字向东[1] 徐华伟[1] 黄磊[1] 穆晓琨[1] 

机构地区：[1]西南民族大学动物遗传育种学国家民委-教育部共建重点实验室,四川成都610041

出　　处：《西南民族大学学报（自然科学版）》2010年第1期80-83,共4页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省科技厅应用基础项目(07JY029-128)资助

摘　　要：扩增出牦牛催乳素受体(PRLR)基因部分保守序列,为研究牦牛乳腺组织中PRLR基因表达水平奠定基础.提取牦牛乳腺组织总RNA,根据NCBI的奶牛PRLR基因cDNA序列的保守区设计特异性引物,采用RT-PCR技术扩增牦牛PRLR基因,结果获得577 bp的片段,将该片段连接于pMD18-Simple质粒中,转化大肠杆菌,菌液PCR鉴定阳性克隆子,测序并分析氨基酸序列.分析序列与氨基酸与其他物种同源性,结果表明该序列与水牛、奶牛、绵羊、人、小鼠的PRLR基因mRNA的对应序列的同源性分别为97.40%、99.13%、93.41%、71.07%、60.20%,编码的氨基酸同源性分别为97.40%、100%、95.83%、82.38%、72.22%.Partial conversed sequence of prolactin receptor （PRLR） gene of yaks is obtained by PCR in order to provide information for studying the expression of PRLR gene in yak mammary tissue. The total mRNA of yak mammary tissue is isolated, and RT-PCR based on the specific primer, which is designed with Software primer 5 according to cow PRLR gene of NCBI. The result shows the sequence is 577 bp. The 577 bp DNA is ligated with Plasmid PMD18-simple, and then transformed into bacillus coll. The positive clones which are identified by PCR （transformed bacillus coli as template） are used for sequencing and deducing amino acid sequence. Comparing the obtained sequence and sequence of amino acids with those in buffalo, cow, sheep, human, rat and mouse respectively, 99.13%, 97.40%, 93.41%, 71.07%, 60.20% in nucleotide homology and 100%, 97.40%, 95.83%, 82.38%, 72.22% in the amino acid homology are observed.

关 键 词：牦牛 催乳素受体 克隆 

分 类 号：S823[农业科学—畜牧学]