ELISA测定血清半胱氨酸蛋白酶抑制剂C  

The Method for Determination of Cystatin C by ELISA

作　　者：单桂芬[1] 林伟[2] 荣墨克[2] 张静春[2] 

机构地区：[1]吉林大学第二医院检验科,吉林长春130041 [2]吉林大学中日联谊医院

出　　处：《中国实验诊断学》2010年第1期105-106,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的建立ELISA方法测定血清半胱氨酸蛋白酶抑制剂C。方法以双抗体夹心ELISA方法测定血清半胱氨酸蛋白酶抑制剂C。结果该方法的线性范围可达100mg/L。参考值范围为:男,1.81±0.73mg/L。女,1.72±0.69mg/L。回收率为:99.1±3.1%。批内和批间分别为:5.8±0.5%、7.3±0.7%。结论该方法操作简便,快速,准确,适于临床常规应用。Objective Th a enzyme-linked inununoserbent assay （ELISA） for measuring eystatin C. Methods e7 ystatin C in serum was measured by sandwieh-type ELISA. Results The linearity of method for up to 100 mg/L. The reference value range was： males, 1.81 ±0.73 mg/L. females, 1.72± 0.69 mg/L. The analytieal recovery was 99.1 ±3.1%. The mean eoeffieients of variation were 5.8±0.5% within-assay and 7.3±0.7% between-assay,respectively. Conclusion The method was simple,rapid and aeeu- rate to operate. It is suitable for routine elinieal labtatory use.

关 键 词：ELISA方法 半胱氨酸蛋白酶抑制剂C 肾功能 

分 类 号：R446.61[医药卫生—诊断学]