低酸度酒精阳性乳患牛乳汁蛋白组分及氨基酸含量变化  

Changes of proteins and amino acids compotents in milk of dairy cows suffered from APM

作　　者：王允田[1] 王振勇[1] 马健[1] 刘操[1] 高媛媛[1] 张西营[1] 陈甜甜[1] 

机构地区：[1]山东农业大学动物医学院,山东泰安271018

出　　处：《中国兽医学报》2010年第1期115-118,共4页Chinese Journal of Veterinary Science

基　　金："华北农区(山东)奶业现代生产技术集成与产业化示范"项目(2002BA518A16);"十五"国家科技攻关奶业重大专项

摘　　要：采用聚丙烯酰胺凝胶电泳和酸水解法对低酸度酒精阳性乳患牛乳汁中蛋白组分及17种氨基酸含量进行了测定。结果显示,酒精阳性乳患牛乳汁中总蛋白、SA、lgG-H、-αLG、-βLG相对百分含量无显著变化(P>0.05),tCN相对百分含量显著降低(P<0.05);在酪蛋白中,-αCN相对百分含量无显著变化(P>0.05),-βCN相对百分含量上升(P<0.05),-κCN相对百分含量极显著下降(P<0.01);17种氨基酸中,Cys含量极显著下降(P<0.01),而Arg含量极显著上升(P<0.01),Leu含量亦呈上升趋势(P<0.05)。结果表明,酒精阳性乳患牛乳汁中主要蛋白组分及氨基酸的含量均发生了变化,乳汁中酪蛋白微球的稳定性遭到了严重破坏,乳汁胶体的稳定性降低,导致酒精试验呈阳性反应。Relative percentage contents of proteins and contents of 17 kinds of amino acids in milk of dairy cows suffered from alcohol positive milk （APM） were detected by means of polyacrylamide gel electrophones and amino acid analyzer. The result indicated that relative percentage contents of TPR,SA,lgG-H,α-LG,β-LG in skim milk of dairy cows suffered from APM were no significant changes （P〉0. 05）, relative percentage contents of total casein（tCN） decreases（P〈0.05）. Relative percentage content of β-CN in casein increased（P〈0.05） and relative percentage content of κ-CN decreased markedly （P〈0.01）. Amino acid analysis data shows that content of Cys decreased markedly （P〈0.01）, but content of Arg increased markedly（P〈0. 01） ,content of Leu increased （P〈0. 05）. Based on the results,The changes of main milk proteins and contents of amino acids of APM destroy the stability of casein microballoons acutely. Decreased stability of milk colloid causes alcohol experiment positive.

关 键 词：酒精阳性乳 乳稳定性 乳蛋白 氨基酸 

分 类 号：S857.2[农业科学—临床兽医学]