10株猪圆环病毒2型(PCV2)河南株全基因组的克隆与序列分析  

Sequence analysis of complete genome of ten porcine circovirus type 2 strains in Henan province

作　　者：陈陆[1] 彭志锋[1] 杨霞[1] 陈圆圆[1] 王宏魁[1] 孙彦婷[1] 郑鹿平[1] 王川庆[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国兽医学报》2010年第2期166-173,共8页Chinese Journal of Veterinary Science

基　　金：国家科技支撑计划资助项目(2006BAK02A21/3);国家"863"计划资助项目(2007AA100606)

摘　　要：为了解猪圆环病毒2型(PCV2)河南株的起源及遗传变异,从河南省不同临床表现的病猪中克隆了10株PCV2的全基因组,并对其进行了序列测定及分析。结果表明,河南株之间以及与中国其他地区PCV2流行株之间同源性均很高;河南株均处于欧洲分支中,提示PCV2河南株可能起源于欧洲株;河南省不同年份的PCV2中确实存在一定的基因差异,但PCV2全基因组从总体上来说遗传进化相对稳定;河南省猪群中与PCV相关的疾病不是由PCV2变异毒株引起的。To understand the genetic heterogeneity and the origin of PCV2 strains in Henan provine, complete genomes of 10 PCV2 strains were cloned and analysed from the swines with Various clinical manifestation in Henan province. The result indicated that the PCV2 strains in Henan wre closely related to each other, but also to PCV2 strains from other areas of China. The PCV2 strains in Henan were included in the Europe cluster, h would be conclued that the PCV2 strains in Henan may originate from Europe PCV2. Although minor genetic differences among PCV2 strains in different years,the genome of PCV2 strains in Henan province exhibited a quite stable genetic homogeneity. The porcine circovirus-associated disease in Henan province is most likely not due to the emergence of a new genotype of PCV2.

关 键 词：猪圆环病毒2型 全基因组克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]