PCR检测临床病例中PCV-2、PRRSV和CSFV的混合感染  

Detection of PCV-2,PRRSV and CSFV mixed infection in clinical cases

作　　者：何后军[1,2] 陆承平[1] 罗咏梅[2] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]江西农业大学动物科技学院,江西南昌330045

出　　处：《中国兽医学报》2010年第2期177-179,共3页Chinese Journal of Veterinary Science

基　　金：江西省科技厅重大招标资助项目(赣科发计字[2005]93号);江西省教育厅资助项目(赣教高字[2008]3号)

摘　　要：参照GenBank中已发表的有关基因序列,分别设计合成针对PCV-2、PRRSV和CSFV的3对引物,分别建立检测临床病例中PCV-2、PRRSV和CSFV感染的PCR或RT-PCR方法。结果显示,扩增产物经琼脂糖凝胶电泳,分别各呈现1条大小约1154,372,375bp的特异条带。采用建立的PCR方法对江西各地发病猪和死亡猪的133份临床病料进行PCV-2检测,结果总检出率53.38%(71/133)。在71份PCV-2阳性的病料中检测出PRRSV20份,阳性率28.17%(20/71);CSFV9份,阳性率12.68%(9/71);PRRSV和CSFV共同感染9份,阳性率12.68%(9/71)。To develop a specific PCR detecting method,three pairs of primers were designed to amplify separately a partial fragment of PCV-2,PRRSV and CSFV, referring to the genome published in Genbank. The PCR or RT-PCR product was separately about 1 154,372 and 375 bp by gelose gel electrophoresis detection. 133 diseased or dead swine specimens collected from different pig farms in Jiangxi were detected by the PCR for PCV-2. It showed 71 specimens were positive for PCV-2, the positive rate was 53. 38%. Meanwhile, the 71 specimens were detected by RT-PCR for PRRSV and CSFV,20 specimens were postive for PRRSV, 9 specimens were postive for CSFV,9 specimens were postive simultaneously for PRRSV and CSFV, the positive rate was separately 28. 17 %, 12.68 % and 12.68 %.

关 键 词：猪圆环病毒2型 猪繁殖与呼吸综合征病毒 猪瘟病毒 混合感染 

分 类 号：S852.65[农业科学—基础兽医学]