α-酮戊二酸对免疫应激下断奶仔猪肝损伤的影响  

Effect of alpha-ketoglutarate on liver injury in weanling pigs with immunologic stress

作　　者：李永塘[1] 侯永清[1] 丁斌鹰[1] 刘玉兰[1] 朱惠玲[1] 王蕾[1] 刘坚[1] 

机构地区：[1]武汉工业学院动物营养与饲料科学湖北省重点实验室,湖北武汉430023

出　　处：《中国兽医学报》2010年第2期239-242,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30871801);湖北省自然科学基金计划创新群体项目(2007ABC009)

摘　　要：24头体质量为(7.25±1.13)kg的(21±1)日龄(杜×长×大)断奶仔猪随机分为3组,每个组设有8个重复,每个重复1头猪。3个组分别为:饲喂基础日粮,注射生理盐水(对照组);饲喂基础日粮,注射脂多糖(LPS)(LPS组);饲喂基础日粮+1%α-酮戊二酸(AKG),注射LPS(AKG组)。预试期7d,正式试验期16d,探讨AKG对免疫应激下断奶仔猪肝损伤的影响。结果显示:(1)LPS刺激导致血清腺苷脱氨酶(ADA)的活性显著升高了15.29%(P<0.05),日粮添加1%AKG血清ADA活性较LPS组降低了7.85%(P>0.05);(2)LPS刺激导致肝脏丙二醛(MDA)活性显著升高了59.61%(P<0.05)以及谷胱甘肽过氧化物酶(GSH-Px)活性显著降低了23.21%(P<0.05),而日粮添加1%AKG肝脏MDA活性较LPS组降低了13.70%(P>0.05)、GSH-Px活性则升高了17.16%(P>0.05);(3)LPS刺激导致肝脏总蛋白质含量和RNA/DNA比值分别显著增加了8.20%(P<0.05),10%(P<0.05),而日粮添加1%AKG较LPS组肝脏总蛋白质含量和RNA/DNA比值分别降低了3.94%(P>0.05),10%(P<0.05)。结果表明:AKG能在一定程度上缓解LPS刺激对断奶仔猪肝脏的损伤。Effects of alpha ketoglutarate on liver injury in weanling pigs with immunologic stress was studied. 24 pigs （Duroc×Landrace × Large White, （7.25 ± 1.13） kg BW of （21 ±1 ）-day-old） were divided into three groups, with 8 replicates of 1 each . The treatment was as following： fed basical diet,injected physiological saline （control group）; fed basical diet, injected LPS （LPS group） ; fed basical diet with 1 %AKG,injected LPS（AKG group）. The trial lasted for 23 days,including 7 days for adaptation. The result showed that （1）LPS increased plasma ADA activity by 15.29%（P〈0. 05）,co-administration with 1%AKG alleviated ADA activity increase by 7. 85% （P〉0. 05）; （2） LPS increased liver MDA activity by 59.61%（P〈0.05）and decreased GSH-Px activity by 23.21% （P〈0.05）, coadministration with 1% AKG decreased MDA activity by 13. 70% （P〉 0. 05）and increased GSH-Px activity by 17.16%（P〉0.05） ;（3）LPS increased liver total protein and the ratio of RNA/DNA by 8.20%（P〈0.05）and 10% （P〈0. 05）, respectively, co-administration with 1% AKG decreased total protein and the ratio of RNA/DNA by 3.94 % （P〉0.05） and 10 % （P〈0.05）, respectively. These results indicated that AKG could to some extent relieve liver injury induced by LPS stress.

关 键 词：Α-酮戊二酸 免疫应激 肝损伤 断奶仔猪 脂多糖 

分 类 号：S852.2[农业科学—基础兽医学]