培养人角朊细胞中白介素15的基因表达  

The Expression of IL 15 mRNA in Cultured Human Keratinocytes

作　　者：韩钢文[1] 岩月氏 马圣清[1] 金子史男 

机构地区：[1]北京医科大学第一医院皮肤科,100034 [2]日本福岛医科大学皮肤科

出　　处：《中华皮肤科杂志》1998年第4期236-238,共3页Chinese Journal of Dermatology

摘　　要：目的　为了探讨白介素 1 5 (IL 1 5 )在表皮角朊细胞中的表达及调节。方法　应用RT PCR及Northern杂交方法分析了IL 1 5在培养的人角朊细胞及鳞状细胞癌细胞系 (HSC 5 )中的表达 ,并观察了地塞米松对角朊细胞表达IL 1 5的影响。结果　正常角朊细胞及鳞状细胞癌细胞系细胞均有IL 1 5mRNA的表达 ,1 0 -6mol/L地塞米松处理培养的角朊细胞后 ,IL 1 5mRNA表达水平明显降低。结论　表皮细胞来源的IL 1Objective To study the expression and regulation of interleukin 15 (IL 15) in epidermal keratinocytes. Methods The expression of IL 15 mRNA in cultured normal human keratinocytes (NHKs) and human squamous cell carcinoma cell line (HSC 5) was analysed, and the effect of dexamethasone on IL 15 mRNA expression was studied by using RT PCR and Northern blotting technique. Results The results showed that both NHKs and HSC 5 expressed IL 15 constitutively. The level of IL 15 mRNA was significantly decreased after the cells were cultured with 10 -6 M dexamethasone. Conclusion It is suggested that keratinocyte derived IL 15 might be involved in the development of certain inflammatory skin diseases.

关 键 词：角蛋白细胞 白细胞介素15 印迹法 RNA 

分 类 号：R392.11[医药卫生—免疫学]