L-半胱氨酸对冻融后猪精子质量参数和膜脂质过氧化反应的影响  

Effects of addition of L-cysteine on quality parameters and membrane lipid peroxidation of frozen-thawed boar sperm

作　　者：张旭成[1] 魏世宝[1] 袁慧敏[1] 金一[1] 

机构地区：[1]延边大学农学院动物科学系,龙井133400

出　　处：《安徽农业大学学报》2010年第1期51-53,共3页Journal of Anhui Agricultural University

基　　金：211工程重点学科项目资助

摘　　要：试验检测冷冻稀释液中添加L-半胱氨酸对冻精解冻后精子质量参数、膜脂质过氧化程度的影响。结果显示:(1)在冷冻稀释液中添加0.1 mg.mL-1L-半胱氨酸能显著地提高冻融后精子的活率、顶体完整率及体外受精率(P<0.05),但对运动学参数没有影响(P>0.05)。(2)在冷冻稀释液中添加L-半胱氨酸(0.1、0.2和0.3mg.mL-1L-半胱氨酸)能显著降低膜脂质过氧化水平(P<0.05)。该结果表明冷冻稀释液中添加L-半胱氨酸能改善猪精子的冷冻效率。The main objective of this study was to examine the effects of addition of L-cysteine in diluents on the qualitative parameters and peroxidation level of membrane lipid of frozen-thawed sperm. The results show that ： （ 1 ） Addition of 0. 1 mg·mL^-1L-cysteine in diluents can significantly improve the viability , the rate of acrosomal integrity and in vitro fertilization rate of frozen-thawed sperm （ P 〈 0. 05 ）, but does not affect the kinematic parameters （P 〉 0.05 ）. （2）Addition of L-cysteine （0. 1,0.2 and 0. 3 mg·mL^-1L-eysteine） in diluents can significantly reduce the level of membrane lipid peroxidation （P 〈 0. 05）. Our results show that addition of L-cysteine in diluents can improve the efficiency of pig sperm cryopreservation.

关 键 词：猪 精子 L-半胱氨酸 质量参数 膜脂质过氧化 

分 类 号：S828.34[农业科学—畜牧学]