血府逐瘀注射液对脑损伤模型大鼠脑组织NF—κB表达与活化的影响  

Effects of xuefuzhuyu injection on expression and activation of NF-κB in rat brain tissue after traumatic brain injury

作　　者：陈兵[1] 尹延庆[1] 彭浩[1] 吴华伟[1] 梁远生[1] 龙霄翱[1] 徐军发[2] 陈东[2] 

机构地区：[1]广东医学院附属医院神经外科,湛江524001 [2]广东医学院检验学院,湛江524023

出　　处：《中华神经医学杂志》2010年第1期15-19,共5页Chinese Journal of Neuromedicine

基　　金：基金项目：广东省医学科研基金（A2008484）;湛江市科技攻关项目（2008C05006）

摘　　要：目的探讨血府逐瘀注射液对脑损伤模型大鼠脑组织核因子-κB（NF-κB）及I-κBα表达的影响。方法80只SD大鼠按随机数字表法分为假手术组（20只）、模型组（20只）、地塞米松组（20只）、血府逐瘀组（20只），采用自由落体撞击法制作大鼠脑损伤模型，假手术组和模型组大鼠腹腔注射0．1mL／（kg·d）生理盐水，地塞米松组及血府逐瘀组大鼠腹腔分别注射地塞米松0．1mg／（kg·d）和血府逐瘀注射液4mL／（kg·d），应用HE染色和免疫组化染色分别观察大鼠脑组织结构和造模后6、24、48、96h脑组织NF—κBp65和I-κBα蛋白的表达。结果与假手术组比较．模型组各时间点NF-κBp65蛋白表达均增高，I-KBα蛋白的表达均降低，差异有统计学意义（P〈0．05）；与模型组比较，血府逐瘀组和地塞米松组大鼠各时间点NF-κB表达均降低，I-κBα的表达均增高，差异有统计学意义（P〈0．05），且血府逐瘀的作用优于地塞米松；模型组大鼠NF—κB与I-KBα的表达呈负相关关系（r=-0．876，P=-0．000）。结论血府逐瘀注射液与地塞米松均可以抑制大鼠脑损伤后脑组织NF—κB的活化，增加I-KBα的表达，减轻创伤性脑损伤后继发性损伤，且血府逐瘀注射液的效果优于地塞米松。Objective To investigate the effects ofxuefuzhuyu injection on the expressions of the nuclear factor-κB （NF-κB） and inhibitor-κBα （I-κBα） in rat brain tissue after traumatic brain injury. Methods Eighty SD rats were randomly divided into sham-operative （n=20）, model （n=20）, hexadecadrol-treated （n=20） and xuefuzhuyu-treated （n=20） groups. Traumatic brain injury models were induced by free-fall. Sham-operative and model groups received intraperitoneal injection of 0.1 mL/（kg·d）normal saline; dexamethasone-treated group and xuefuzhuyu-treated group received intraperitoneal injection of 0.1 mg/（kg·d） dexamethasone and 4 mL/（kg·d） xuefuzhuyu injection immediately after the successful model inducement, respectively, and then, heir brain tissue samples were obtained at 6, 24, 48 and 96 h. lmmunohistochemistry was employed to detect the protein expressions of NF-κB P65 and I-κBα in the traumatic brain tissue, and HE staining was performed to observe the brain structure. Results Increased protein expression of NF-κB P65 and decreased protein expression of I-KBα in the model group were found as compared to those in the sham-operative group at all time points （P〈0.05）; dexamethasone-treated and xuefuzhuyu-treated groups showed significantly lower protein expression of NF-κB and higher protein expression of I-κBα than the model group at all time points （P〈0.05） and the effect of xuefuzhuyu injection was better than that of dexamethasone. The expressions of NF-κB and I-KBα were negatively correlated in the model group （r=-0.876, P=0.000）. Conclusion Secondary injury caused by traumatic brain injury can be relieved by inhibiting the activation of NF-κB and increasing the expression of I-κBα, which are exactly the effects of xuefuzhuyu injection and dexamethasone, and xuefuzhuyu injection works better than dexamethasone.

关 键 词：颅脑损伤 核因子-ΚB I-ΚBΑ 地塞米松 血府逐瘀注射液 

分 类 号：R285.5[医药卫生—中药学]