脑室内注射碱性成纤维细胞生长因子治疗大鼠创伤性脑外伤的实验研究  

Intraventricular injection of basic fibroblast growth factor in rats with traumatic brain injury

作　　者：王孝安[1] 刘卫平[1] 刘阳[2] 玉石[1] 龙乾发[1] 

机构地区：[1]第四军医大学西京医院神经外科,西安710032 [2]绵阳市第三人民医院神经外科,621000

出　　处：《中华神经医学杂志》2010年第1期24-28,共5页Chinese Journal of Neuromedicine

摘　　要：目的探讨脑室内注射碱性成纤维细胞生长因子（bFGF）对创伤性脑外伤大鼠是否具有治疗作用。方法24只成年SD大鼠按随机数字表法分为治疗组和对照组，采用改良的Feeney氏自由落体撞击方法建立大鼠创伤性脑外伤模型，致伤后24h分别给予脑室内注射bFGF和等量的生理盐水；采用行为测试试验（前肢放置试验、平衡试验）评分观察肢体功能恢复情况；采用免疫组化法，以5．溴脱氧嘧啶尿苷（Brdu）标记神经干细胞，观察并比较两组大鼠致伤后第3、7、14天侧脑室室管膜下区（SVZ）、海马齿状回及损伤区域Brdu阳性细胞的表达。结果治疗组前肢放置试验评分在第3～12天、平衡试验在第3～11天的评分低于对照组，比较差异有统计学意义沪〈0．05）。治疗组双侧SVZ、海马齿状回和损伤区域出现的Brdu阳性细胞数较对照组明显增多，差异有统计学意义（P〈0．05）。两组损伤侧Brdu阳性细胞数均高于损伤对侧，比较差异有统计学意义俨〈0．05）。行为学评分与Brdu阳性细胞数之间呈负相关关系。结论脑室内注射bFGF有助于创伤性脑外伤大鼠模型内源性神经干细胞的增殖，并能促进其肢体功能的恢复。Objective To investigate the effect of basic fibroblast growth factor （bFGF） by intraventricular injection on traumatic brain injury （TBI） in rats. Methods Twenty-four adult SD rats were randomly divided into treatment group and control group. TBI model was established by modified Feeney＇s method. The treatment group and the control group were intraventricularly injected bFGF and saline 24 h after TBI, respectively. The recovery of limb function was observed by behavior tests （forelimb placing test and beam balance test）. After labeling the neural stem cells with bromodeoxyuridine （Brdu）, irnmunohistochemical staining, was employed to measure and compare the expression of Brdu-positive cells in the lateral subventricular zone （SVZ）, the dentate gyrus of the hippocampus and the damage region at 3, 7 and 14 d after TBI. Results The grading of forelimb placing test and beam balance test in the 2 groups was significantly different： in the forelimb placing test, statistical significance was found from the 3rd to the 12th d （P〈0.05）; in the beam balance test, statistical significance was found from the 3rd to the 1 lth d （P〈0.05）. Compare with the control group, treatment group showed significantly increased number of Brdu-positive cells in the bilateral SVZ, the dentate gyrus of the hippocampus and the damage region after TBI （P〈0.05）. The number of Brdu-positive cells on the damage side was significantly higher than that on the opposite side （P〈0.05）. The grading of behavior test was negatively correlated to the number of Brdu-positive cells. Conclusions Intraventricular injection of bFGF can increase the proliferation of endogenous neural stem cells and speed up the recovery of limb function in TBI model of rats.dogenous neural stem cells and expidite the restoration of limbs fimction in the rat model of TBI.

关 键 词：颅脑损伤 碱性成纤维细胞生长因子 

分 类 号：R651.15[医药卫生—外科学]