毛囊混合细胞植入胶原／壳聚糖多孔支架诱导裸鼠毛发生长及支架内形成血管样结构  

Potency of hair follicle bulb cells implanted into collagen/chitosan porous scaffolds to induce the growth of hair and formation of vessel-like structure in scaffolds

作　　者：吴贤杰[1] 吴巧云[1] 高欣[2] 郑敏[1] 吕中法[1] 蔡绥勍[1] 周燕[2] 马列[3] 高长有[3] 

机构地区：[1]浙江大学医学院附属第二医院皮肤科,杭州310009 [2]浙江大学医学院附属第二医院病理科,杭州310009 [3]浙江大学材料与化工学院

出　　处：《中华皮肤科杂志》2010年第1期14-17,共4页Chinese Journal of Dermatology

基　　金：浙江省医药卫生科技计划（2007A090）;浙江省医学会临床科研基金（2008ZYC11）

摘　　要：目的探讨利用毛囊混合细胞植入胶原／壳聚糖多孔支架内重建毛囊的可行性与支架内血管形成状态。方法用注射法将体外培养的C57BL／6J近交系乳鼠背部皮肤毛囊混合细胞接种至胶原境聚糖多孔支架，培养2周后，将含毛囊混合细胞胶原／壳聚糖多孔支架植入裸鼠皮下，肉眼观察裸鼠背部毛发形成情况。6周后取移植区皮肤组织经10％甲醛固定后行组织学观察（HE染色）。结果移植至裸鼠皮下5周后，裸鼠背部在含毛囊混合细胞的胶原／壳聚糖多孔支架移植区皮肤出现毛发生长，6周后取皮肤组织作HE染色发现有分化成熟的毛囊形成，且支架内有血管样结构形成。而空白胶原／壳聚糖多孔支架移植区皮肤未发现毛生长，组织检查也未发现有毛囊形成，且只有在支架表浅部位有少量血管样结构形成。结论毛囊混合细胞植入胶原／壳聚糖多孔支架内可诱导裸鼠毛发的形成，促进支架血管样结构的形成。Objective To investigate the possibility of hair follicle reformation induced by hair follicle bulb cells implanted into collagen/chitosan porous scaffolds in vivo, and to observe the angiogenesis in implanted scaffolds. Methods Hair follicle bulb cells obtained by enzyme digestion from the back skin of C57BL/6J mice were implanted into collagen/chitosan porous scaffolds followed by 2-week organotypic culture. Then, these collagen/chitosan porous scaffolds were transplanted subcutaneously into the dorsal skin of nude mice. Those nude mice transplanted with empty collagen/chitosan porous scaffolds served as the controls. The growth of hair was observed with naked eyes. Six weeks after the transplantation, skin samples were obtained from the recipient site and subjected to histological examination. Results Five weeks after the transplantation, hair growth was observed in the dorsal skin of nude mice. Six weeks later, histological examination revealed fully differentiated hair follicles and vessel-like structures in the center of collagen/ehitosan porous scaffolds. However, the transplantation with empty collagen/chitosan porous scaffolds failed to elicit the same response. No hair or follicles were observed in the control mice along with small number of vessel-like structures. Conclusions Hair follicle bulb cells implanted into collagen/chitosan porous scaffolds in vivo could induce hair follicle reformation and promote the formation of vessel-like structure in the scaffold center.

关 键 词：毛囊 胶原 壳聚糖 血管 

分 类 号：R318.08[医药卫生—生物医学工程]