珍稀植物跳舞草的组织培养与快速繁殖  

Tissue Culture and Rapid Propagation of Rare Plant in Codariocalyx motorius(Houtt.)

作　　者：毛艳萍[1,2] 苏智先 邹利娟 吴庆贵 

机构地区：[1]西华师范大学生命科学学院,南充637002 [2]四川省生态与环境技术重点实验室,绵阳621000

出　　处：《植物研究》2010年第1期106-110,共5页Bulletin of Botanical Research

基　　金：国家自然科学基金(NO.30670209)

摘　　要：以跳舞草无菌苗为实验材料,以MS、1/2MS为基本培养基,研究不同条件对跳舞草快速繁殖的影响,初步建立了跳舞草组培快繁体系,筛选出最佳愈伤组织诱导及不定芽分化培养基为MS+6-BA2.0mg·mL-1+NAA0.1mg·mL-1+蔗糖30.0g.L-1+VC2.0mg·mL-1,最佳增殖培养基为MS+6-BA2.0mg·mL-1+NAA0.05mg·mL-1,最佳生根培养基为1/2MS+NAA0.2mg·mL-1+IAA0.5mg·mL-1。The aseptic seedings of Codariocalyx motorius（Houtt.）Ohashi were the materials of our study on issue culture and rapid propagation,MS and 1/2MS were the basic mediums.Impact of different conditions on the rapid propagation of C.motorius（Houtt.）was discussed and a tissue culture system of C.motorius（Houtt.）was initially established,the best callus adventitious and bud differentiation medium was MS＋6-BA 2.0 mg·L-1＋NAA 0.1 mg·L-1＋sugar 30 g·L-1＋VC 2.0 mg·L-1,the best medium for the shoot regeneration was MS＋6-BA 2.0 mg·L-1＋NAA 0.05 mg·L-1,the best rooting medium was 1/2MS＋NAA 0.2 mg·L-1＋IAA 0.5 mg·L-1.

关 键 词：跳舞草 不定芽增殖 组织培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]