宁夏枸杞遗传多样性的RAPD分析  

Genetic Diversity Analysis of Lycium barbarum L. by RAPD

作　　者：尚洁[1] 李收[1] 张靠稳[1] 

机构地区：[1]北方民族大学生物科学与工程学院,银川750021

出　　处：《植物研究》2010年第1期116-119,共4页Bulletin of Botanical Research

基　　金：北方民族大学资助项目(2006Y040)

摘　　要：利用13个经筛选在样品间具多态性的10碱基随机引物,对宁夏枸杞(Lycium barbarum L.)4个主要栽培品种及3个宁夏野生枸杞的总DNA进行PCR扩增。在样品间共产生123条RAPD标记带,其中多态性带68条,多态性百分比为55.28%;以Ntsyspc2.1软件计算样品间的遗传相似系数,并进行UPGMA聚类分析。结果表明宁夏枸杞栽培品种间遗传关系较近,特别是宁杞1号和宁杞2号,相似性系数达81.82%;而野生枸杞与栽培品种间遗传关系较远。By screening 13 samples being polymorphic with 10 base random primers,we amplified the genomic DNAs of four domestic and three wild cultivars of Lycium barbarum L..A total of 123 RAPD bands was detected,68 among which were polymorphic,and the percent of polymorphic bands was 55.28%.Then we calculate the genetic similarity coefficient by Ntsyspc 2.1 and did UPGMA cluster analysis,and found the genetic relationship among the domestic cultivars is close,especially Ningqi No.1 and Ningqi No.2,whose similarity coefficient goes 81.82%,but between the domestic cultivars and the wild cultivars the genetic relationship is far.

关 键 词：宁夏枸杞 遗传多样性 RAPD 

分 类 号：Q346[生物学—遗传学]