Goldfish β-catenin cell-autonomously inhibits the expression of early neural development regulating gene vsx1  

作　　者：ZHANG QiongYu ZHENG Kang MA ShanShan TONG Ying LUO Chen 

机构地区：[1]College of Life Science, Hunan Normal University, Changsha 410081, China [2]College of Life Science, Zhejiang University, Hangzhou 310058, China

出　　处：《Chinese Science Bulletin》2010年第2期145-152,共8页

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30430370);National Key Basic Research and Devel-opment Program of China (Grant No. 2004CB117401)

摘　　要：β-catenin gene is essential for the formation of normal dorsal axial structure in vertebrates. Recent studies have provided evidence that β-catenin has a certain role in restricting the amount of organizer-induced neurectoderm formation in zebrafish and Xenopus laevis. To further understand how β-catenin represses the induction of neurectoderm formation and whether the inhibition of neural progenitor fates is essential for the normal organizer formation, it was investigated whether β-catenin was involved in repressing the early neural regulatory gene Visual system homeobox-1 (vsx1) expression during embryogenesis. It was observed that functional inhibition of endogenous β-catenin by morpholino oligonucleotides (MO) resulted in extensive and ubiquitous vsx1 expression, β-catenin could repress vsx1 promoter-driven GFP expression and transcriptional factor gene boz, the direct target gene of β-catenin, could directly repress vsx1 transcription by binding to vsx1 proximal promoter. These observations indicate that β-catenin can repress vsx1 ectopic expression cell-autonomously while it activates chordamesodermal genes expression in the precursory cells of chordamesoderm, suggesting both activation of chordamesodermal genes and repression of neural regulatory genes vsx1 in precursory chordamesoderm by β-catenin are essential for normal notochord formation.β-catenin gene is essential for the formation of normal dorsal axial structure in vertebrates. Recent studies have provided evidence that β-catenin has a certain role in restricting the amount of organizer-induced neurectoderm formation in zebrafish and Xenopus laevis. To further understand how β-catenin represses the induction of neurectoderm formation and whether the inhibition of neural progenitor fates is essential for the normal organizer formation, it was investigated whether β-catenin was involved in repressing the early neural regulatory gene Visual system homeobox-1 （vsxl） expression during embryogenesis. It was observed that functional inhibition of endogenous β-catenin by morpholino oligonucleotides （MO） resulted in extensive and ubiquitous vsxl expression, 1%catenin could repress vsxl promoter-driven GFP expression and transcriptional factor gene boz, the direct target gene of β-catenin, could directly repress vsxl transcription by binding to vsxl proximal promoter. These observations indicate that β-catenin can repress vsxl ectopic expression cell-autonomously while it activates chordamesodermal genes expression in the precursory cells of chordamesoderm, suggesting both activation of chordamesodermal genes and repression of neural regulatory genes vsxl in precursory chordamesoderm by β-catenin are essential for normal notochord formation.

关 键 词：神经前体细胞 Β-连环素 神经调节 基因表达 早期 转录因子基因 金鱼 胚胎发育过程 

分 类 号：Q421[生物学—神经生物学] Q26[生物学—生理学]