Study of alternate cooling and heating treatment induced tumor microvasculature injury  

作　　者：SHEN YuanYuan ZHANG AiLi 

机构地区：[1]Department of Biomedical Engineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Chinese Science Bulletin》2010年第2期172-178,共7页

基　　金：supported by the National Natural Science Foundation of China (Grant No. 50725622);Shanghai Engineering Technical Research Centre (Grant No. 08DZ2211201);National Basic Research Program of China (Grant No. 2009CB930403)

摘　　要：Tumor microvasculature is important to tumor growth, metastasis and thus tumor treatment outcome. The alternate cooling and heating treatment has been confirmed to have advantages over single treatment of cooling or heating. The degree of tumor microvasculature damage induced by the alternate cooling and heating treatment and the mechanisms underlying are studied in this paper. The response of the tumor microvasculature to different treatments including alternate cooling and heating is observed in vivo through confocal microscopy using the nude mice dorsal skin fold tumor chamber model. Results show that alternate cooling and heating has induced much more severe damage to the microvessel structure throughout the entire tumor. Numerical simulations of the mechanical stresses on the tumor vessel wall has found that during the alternate treatment, the vessel wall suffer a rapid chang in thermal stresses in the opposite directions successively, which might caused damage to the peripheral microvasculature and micro-cracks in the central vessels. Reperfusion of blood flow after freezing also led to relatively large stresses on the vessel wall, especially when blood flow re-perfuses quickly during the subsequent heating. The quick increase of stresses on the blood vessel might be one of the key issues causing the blood vessel rupture referring to the experimental observation. The preliminary study has partly revealed the mechanism underlying serious tumor microvasculature damage caused by the alternate cooling and heating treatment.Tumor microvasculature is important to tumor growth, metastasis and thus tumor treatment outcome. The altemate cooling and heating treatment has been confirmed to have advantages over single treatment of cooling or heating. The degree of tumor microvasculature damage induced by the altemate cooling and heating treatment and the mechanisms underlying are studied in this paper. The response of the tumor microvasculature to different treatments including alternate cooling and heating is observed in vivo through confocal microscopy using the nude mice dorsal skin fold tumor chamber model. Results show that alternate cooling and heating has induced much more severe damage to the microvessel structure throughout the entire tumor. Numerical simulations of the mechanical stresses on the tumor vessel wall has found that during the alternate treatment, the vessel wall suffer a rapid chang in thermal stresses in the opposite directions successively, which might caused damage to the peripheral microvasculature and micro-cracks in the central vessels. Reperfusion of blood flow after freezing also led to relatively large stresses on the vessel wall, especially when blood flow re-perfuses quickly during the subsequent heating. The quick increase of stresses on the blood vessel might be one of the key issues causing the blood vessel rupture referring to the experimental observation. The preliminary study has partly revealed the mechanism underlying serious tumor microvasculatare damage caused by the alternate cooling and heating treatment.

关 键 词：肿瘤治疗 血管损伤 过冷却 热诱导 加热处理 血流灌注 显微镜观察 微血管 

分 类 号：Q2[生物学—细胞生物学] Q513