四种子囊菌甲醇提取物的抗氧化活性研究  

Antioxidant activities of the methanolic extracts of four species of Ascomycetes

作　　者：武守华[1] 张晓君 张平[1] 陈作红[1] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081 [2]湖南益康生物高科技有限公司,长沙410329

出　　处：《菌物学报》2010年第1期113-118,共6页Mycosystema

基　　金：湖南省教育厅重点项目(No.08A044)

摘　　要：比较了冬虫夏草Cordyceps sinensis、古尼虫草Cordyceps gunnii、蛹虫草Cordyceps militaris、黑柄炭角菌Xylaria nigripes等四种子囊菌提取物的抗氧化活性,对它们的子实体(F)和菌丝体(M)甲醇提取物清除DPPH自由基能力、还原力、金属离子鳌合能力和总酚含量进行了测定,结果显示四种子囊菌子实体的自由基清除能力、还原力和总酚含量均高于其菌丝体(P<0.05),但四种子囊菌子实体的DPPH清除能力不存在显著差异(P>0.05);古尼虫草和蛹虫草子实体的还原力和总酚含量显著高于冬虫夏草和黑柄炭角菌子实体(P<0.05),但前两者之间不存在显著差异(P>0.05)。总酚含量和DPPH自由基清除能力、还原力呈正相关。The methanol extracts of the mycelium （M） and fruiting body （F） of Cordyceps sinensis, Cordyceps gunnii, Cordyceps militaris and Xylaria nigripes were evaluated for their antioxidative activities using three different assays, 1,1-diphenyl-2-picrylhydrazyl （DPPH） radical scavenging activity, reducing and chelating ability, and the content of total phenols. The results showed that the DPPH radical scavenging activity, reducing ability, and the content of total phenols of the fruiting bodies were significantly higher than those of mycelia in four species of ascomycetes （P〈0.05）, but the fi＇uiting bodies of the species evaluated showed no significant difference in their DPPH scavenging ability （P〉0.05）; Cordyceps gunnii （F） and Cordyceps militaris （F） were significantly higher than Cordyceps sinensis （F） and Xylaria nigripes （F） in their reducing ability and the content of total phenolic （P〈0.05）, but the former two species didn＇t show significant difference （P〉0.05）. The DPPH radical scavenging activity, and reducing ability of the crude extracts is correlated with the content of total phenols.

关 键 词：虫草 黑柄炭角菌 抗氧化 自由基 

分 类 号：S567.3[农业科学—中草药栽培]