深低温冻存毛囊的实验研究  

Experimental study of hair follicle with cryopreservation

作　　者：谭挺[1] 胡志奇[1] 

机构地区：[1]南方医科大学南方医院整形外科,广东广州510515

出　　处：《中国美容整形外科杂志》2010年第1期54-57,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金资助项目（30872692）;广东省自然科学基金资助项目（8151051501000037）;广州市科技计划项目（200723-E0021）

摘　　要：目的建立一种长时间体外保存毛囊的方法。方法取自愿捐献的成人头皮标本，在湿微镜下分离成毛囊单位，随机分为3组。一组直接行毛囊培养；另外两组分别用常规冻存法和玻璃化冻存法液氮中保存21d，复温后行毛囊培养，观测各组毛囊存活及生长情况。结果玻璃化冻存组的毛囊解冻后，其存活及生长情况与直接培养组相近，优于常规冻存组。结论使用玻璃化冻存法长时间保存毛囊，对其存活和特性无明显影响，为临床应用提供了实验基础。Objective To establish a long time conservancy method of hair follicles in vitro. Meth ods The hair follicle were separated from adult human scalp by micromanipulation. The follicles were random- ly allocated to routine cultured, conventional slow freezing and vitrification groups. The freezing two groups were preserved in liquid nitrogen for 21 days. Then they were thawed and cultured. The length of hair follicle and its growth rate were measured and calculated, and its viability was observed. Results The vitrification group showed the same characteristics of viability and growth rate with the routine cuhured group, but it was better than those in the conventional slow freezing groups. Conclusion There influences of vitrification and cryoprsesrvation on the hair follicles＇ viability and characteristics were not significant. This method laid a foundation for the clinical application.

关 键 词：毛囊 玻璃化 深低温冷冻 

分 类 号：R318.52[医药卫生—生物医学工程]