菌落多重PCR鉴定不同荚膜血清型的多杀性巴氏杆菌  

Species Identification and Capsular Serotyping of Pasteurella multocida by Colony Multiplex PCR

作　　者：李伟杰[1] 赵耘[1] 杜昕波[1] 康凯[1] 陈敏[1] 

机构地区：[1]中国兽医药品监察所,北京100086

出　　处：《动物医学进展》2010年第1期6-9,共4页Progress In Veterinary Medicine

基　　金：国家科技支撑计划(项目编号:2006BAD06A11)

摘　　要：参照文献报道的多杀性巴氏杆菌KMT1基因和荚膜生物合成位点hyaD-hyaC、bcbD、dcbF、ecbJf、cbD基因的序列合成了6对特异性引物,建立了多杀性巴氏杆菌种和型的菌落多重PCR方法。结果表明,本所保藏的A、B、D、E、F各型多杀性巴氏杆菌均扩增出了相应的预期片段,PCR结果与Biolog鉴定结果和Carter氏间接血球凝集试验结果相一致;而支气管败血波氏杆菌、胸膜肺炎放线杆菌、大肠埃希菌、猪链球菌和粪肠球菌的扩增均为阴性。Six pairs of primers against species-specific KMT1 gene and serogroup-specific hyaD hyaC,bcbD, dcbF,ecbJ, fcbD genes were designed and the colony multiplex PCR of identification and serogroup of Pasteurella rnultocida were developed. Pasteurella multocida strains stored in China Institute of Veterinary Drug Control including A, B, D, E and F were serotyped using the colony multiplex PCR assay. The results were compared with Biolog and Cater indirect hemagglutination test which showed good accordance. The expected sequences were obtained successfully by the colony muhiplex PCR assay. But the sequences were not obtained from Bordetella bronchiseptica, Actinobacillus pleuropneumoniae, Escherichia coli , Streptococcus suis and Enterococcus f aecalis.

关 键 词：多杀性巴氏杆菌 菌落多重PCR 荚膜血清型 Biolog鉴定 Carter氏间接血凝试验 

分 类 号：S852.612[农业科学—基础兽医学] Q789[农业科学—兽医学]