羧甲基壳聚糖磁性纳米粒子的合成及应用  

Synthesis and Application of Carboxymethyl Chitosan Magnetic Nanoparticles

作　　者：李玉慧[1] 彭婷婷[1] 张丽[1] 欧阳丽[1] 谢青季[1] 苏孝礼[1] 马铭[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,长沙410081

出　　处：《应用化学》2010年第1期87-91,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金重大研究计划(90713018);国家自然科学基金(20875029);湖南省科技计划(2008FJ3079)资助项目

摘　　要：通过合成油酸修饰的Fe3O4纳米粒子和羧甲基壳聚糖直接包埋油酸修饰的Fe3O4纳米粒子的两步合成法制备了羧甲基壳聚糖磁性纳米粒子。采用透射电子显微镜、傅里叶变换红外光谱、振动样品磁强计和同步热分析测试技术对制备的羧甲基壳聚糖磁性纳米粒子进行了表征。所得磁性纳米粒子呈规则球形,粒径约为10 nm;表面含羧基,且具有很好的顺磁性和稳定性。考察了羧甲基壳聚糖磁性纳米粒子对阿霉素的载药量和对阿霉素在磷酸盐缓冲溶液中的缓释性能。结果表明,磁性纳米粒子对阿霉素展示了较高的载药量(91.8 mg/g),结合了阿霉素的磁性复合物对阿霉素的缓释作用明显,说明制备的羧甲基壳聚糖磁性纳米粒子有望作为治疗肿瘤的纳米磁靶向药物输送载体。Carboxymethyl chitosan magnetic nanoparticles（CMCS-OA-Fe3O4 NPs） were prepared by a two-step method,which involved,synthesizing nanosized Fe3O4 in the presence of oleic acid（OA-Fe3O4 NPs） and directly coating the OA-Fe3O4 NPs with carboxymethyl chitosan in aqueous solution.The prepared CMCS-OA-Fe3O4 NPs were characterized with transmission electron microscopy（TEM）,vibrating sample magnetometer（VSM）,Fourier transform infrared spectrometry（FT-IR） and simultaneous thermal analyzer（STA）,respectively.The CMCS-OA-Fe3O4 NPs were found to be regularly spherical with carboxyl on their surface,with a diameter of roughly 10 nm.The CMCS-OA-Fe3O4 NPs exhibited good paramagnetism and stability.The drug loading and the sustained-release property of the CMCS-OA-Fe3O4 NPs for adriamycin（ADM） in phosphate buffer solution（PBS,pH=7.4） were studied.High drug loading（91.8 mg/g） of CMCS-OA-Fe3O4 NPs and obvious sustained-release effect in vitro for ADM were reached.The results indicate that CMCS-OA-Fe3O4 NPs may be used as a magnetic-targeted nano drug carrier for the treatment of tumour.

关 键 词：羧甲基壳聚糖 磁性纳米粒子 释药性能 阿霉素 

分 类 号：O636.1[理学—高分子化学]