非小细胞肺癌血清标志蛋白HSP90α的鉴定及其临床意义  

Serum HSP90α in the clinical stage of non-small cell lung cancer

作　　者：黄凌云[1] 徐安健[2] 蒋单懿[1] 郝佳[1] 谷俊朝[2] 肖雪媛[1] 何大澄[1] 

机构地区：[1]北京师范大学细胞增殖及调控教育部重点实验室,北京100875 [2]首都医科大学附属北京友谊医院,北京热带医学研究所,北京100050

出　　处：《国际外科学杂志》2010年第1期24-28,共5页International Journal of Surgery

基　　金：国家重点基础研究计划（No.2006CB910100）;北京自然科学基金（No.7051002）;北京市科学技术委员会基金（No.Y0204002040111） 致谢 台湾癌症研究所吴成文教授和国立台湾师范大学王忆卿教授惠赠CL1-0和CL1-5细胞系.

摘　　要：目的证明HSP90α是否可以作为肺癌诊断的血清标志蛋白。方法利用SDS—PAGE和基质辅助解吸附时间飞行质谱（MALDI—TOF—MS），对具有不同转移能力的肺腺癌细胞系CL1-0（低转移）和CL1-5（高转移）的培养基上清进行分析，并对已鉴定的候选标志蛋白通过免疫印迹方法进行确定。此外，利用酶联免疫吸附技术（ELISA）对141例肺癌、37例良性肺病患者、及46例健康人血清对候选蛋白进行了进一步的分析。结果HSP90α在CL1—5的培养基中高表达，进一步发现HSP90α在非小细胞肺癌的血清中特异性升高。当ROC曲线（receiver operating characteristic，ROC）临界值取为0．535时，HSP90α能区分肺癌以及良性肺病患者和健康人，其敏感性为0．817，特异性为0．919，总体精确性为80．14％。结论HSP90α有望成为区分肺癌、良性肺病患者和健康人以及肺癌分期诊断的血清标志蛋白。Objective To investigate whether HSP90α could be a sensitive and specific serum biomarker for the diagnosis and progression of lung cancer. Methods In the present study, different secretomic analyses on the two human lung adenocarcinoma cell lines CL1-0 and CL1-5 with low and high metastalic potential, respectively, were performed using sodium dodecyl sultate-polyacrylamide gel electropboresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The candidate biomarker was confirmed by Western blotting, and was further analyzed in 224 sernm samples ineluding 141 lung cancer, 37 benign pulmonary diseases, as well as 46 healthy individuals using ELISA assay. Results HSP90α was significantly upregulated in the CM of CL1-5 ceils, it was found that the levels of HSP90α were specifically elevated in the sera of non-small cell lung cancer compared with other groups. At the cut-off point 0. 535 on the receiver operating oharacteristic curve, HSP90α could comparatively discriminate lung cancer from benign lung disease and healthy control groups with sensitivity of 0. 817, specificity 0. 919 and total accuracy 80.14%. Conclusion HSP90α may be a potential useful serum biomarker for discriminating lung cancer from benign lung diseases and healthy individuals and staging of non-small cell lung cancer.

关 键 词：HSP90Α CL1-0 CL1—5 分泌蛋白质组 肿瘤转移 

分 类 号：R734.2[医药卫生—肿瘤]