柚皮苷分子印迹传感器的制备与应用  

Preparation and Application of Naringin Sensor Based on Molecularly Imprinting Technique

作　　者：马秀玲[1] 陈日耀[1] 郑曦[1] 陈晓[1] 陈震[1,2] 

机构地区：[1]福建师范大学化学与材料学院,福州350007 [2]宁德师范高等专科学校化学系,宁德352100

出　　处：《分析化学》2010年第1期100-104,共5页Chinese Journal of Analytical Chemistry

基　　金：福建省自然科学基金项目(No.D0710009);福建省发展与改革委员会项目(No.2008-762)资助

摘　　要：以邻氨基酚为单体,无电化学活性的柚皮苷为模板分子,采用循环伏安法(扫速为100mV/s)在碳电极上往复扫描30次,电聚合出具有识别柚皮苷分子功能的敏感膜。以扫描电子显微镜(SEM)、X射线全反射(XRR)及电化学方法对该印迹传感器进行表征。结果表明,印迹传感器敏感膜与非印迹膜在形貌结构和电化学特性方面有明显的不同。此传感器对柚皮苷有较好的选择性,响应快(30s),在6.0×10-5～1.4×10-4mol/L范围内呈线性关系,且重现性好(RSD=1.8%,n=5);传感器对柚皮苷的检出限为1.6×10-5mol/L。The sensor of naringin（NG）,a non-electroactive substance,was prepared based on the molecular imprinting technique.Using cyclic voltammetry technique（scan rate is 100 mV/s）,the naringin imprinting sensitive film,poly-ο-aminophenol was coated on the surface of a graphite electrode in the presence of naringin which was considered as the template,and characterized by SEM and X-ray reflective spectrophotometry （XRR）.Using K3Fe（CN）6 as an electroactive marker,the electrochemical properties of the NG sensor were investigated by CV,electrochemical impedance spectroscopy （EIS）,differential pulse voltammmetric and chronoamperometric.The results showed that the imprinted electrode was significantly different from the nonimprinted electrode in morphologies and electrochemical properties,and a linear relationship between the peak current and the naringin concentration was found in the range of 6.0 × 10-5-1.4 × 10-4 mol/L with a detection limit of 1.6 × 10-5 mol/L.Moreover,the imprinted electrode exhibited a good selectivity and rapid response to the naringin template molecules,as well as an excellent reproducibility（RSD=1.8%,n=5）.

关 键 词：柚皮苷 分子印迹 传感器 邻氨基酚 

分 类 号：R318.6[医药卫生—生物医学工程]