基于Nafion/碳纳米粒子修饰的葡萄糖传感器  

Amperometric Glucose Biosensor Based on Carbon Nanoparticles

作　　者：汪美芳[1,2] 张伟[1] 方宾[1] 

机构地区：[1]安徽师范大学化学与材料科学学院,芜湖241000 [2]皖南医学院化学教研室,芜湖241000

出　　处：《分析化学》2010年第1期125-128,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20675001)资助课题

摘　　要：采用滴涂法制备了Nafion/碳纳米粒子复合物修饰玻碳电极,该电极对H2O2具有良好的电催化氧化性能。还利用滴涂法制备了Nafion/碳纳米粒子复合物包裹的葡萄糖酶电化学生物传感器,该生物传感器对葡萄糖有着良好的电催化作用。应用该传感器对葡萄糖进行了检测,检测线性范围为2.0×10-6～6.0×10-3mol/L,检出限为1.6×10-6mol/L(S/N=3),实验结果表明该传感器具有良好的稳定性、重现性和抗干扰能力。对小鼠血清样品中的葡萄糖进行检测,结果令人满意。The nafion/carbon nanoparticles（CNPs） composite film modified glassy carbon electrode was prepared by dropwise method,and the electrocatalyst of H2O2 at Nafion/CNPs modified electrode was investigated.The results show that electrocataytic activitie for detection of H2O2 at the modified electrode is very good.The biosensing application of CNPs was demonstrated through fabrication of an electrochemical biosensor.The biosensor was constructed by encapsulating glucose oxidase in the Nafion/CNPs composite film.The biosensor had good electrocatalytic activity toward oxidation of glucose.The glucose biosensor shows a linear range from 2.0 × 10^-6 to 6.0 × 10^-3 mol/L with a detection limit of 1.6 × 10^-6 mol/L.The biosensor shows high stability,good reproducibility and can avoid the commonly coexisted interference.In addition,real rat serum samples were analyzed by this biosensor with satisfactory results.

关 键 词：碳纳米粒子 过氧化氢 葡萄糖 电化学传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]