VDR基因多态性与HBV宫内传播的相关性研究  

A study on association between VDR gene polymorphisms and HBV intrauterine infection

作　　者：葛婕[1] 韩国荣[2] 

机构地区：[1]东南大学临床医学院,江苏南京210009 [2]东南大学临床医学院附属南京第二医院妇产科,江苏南京210003

出　　处：《中国妇幼健康研究》2010年第1期26-29,共4页Chinese Journal of Woman and Child Health Research

摘　　要：目的探讨维生素D受体基因FokⅠ和TaqⅠ多态性是否与乙型肝炎病毒发生宫内传播之间存在关联，确定乙型肝炎病毒宫内传播的遗传易感基因和保护基因。方法随机选取临床上已证实发生乙型肝炎病毒宫内传播的儿童24例和未发生乙型肝炎病毒宫内传播的儿童20例作为研究对象，收集股静脉血，提取基因组DNA，应用聚合酶链反应-限制性片段长度多态性方法，分析维生素D受体基因第2外显子FokⅠ位点和第9外显子TaqⅠ位点的多态性分布在两组间的差别。结果宫内传播组的FokⅠ f等位基因频率为52．08％，显著高于非宫内传播组（27．50％）,Х^2=12．36，P〈0．05。宫内传播组的TaqⅠ T等位基因频率为97．92％，显著高于非宫内传播组（85．00％）,Х^2=10．87，P〈0．05。非宫内传播组的维生素D受体FokⅠ的FF、Ff及ff基因型频率分别为50．00％、45．00％和5．00％，与宫内传播组的16．67％、62．50％和20．83％相比差异有统计学意义（Х^2=6．72，P〈0．05）。非宫内传播组的维生素D受体TaqⅠ的TT、Tt基因型频率分别为70．00％、30．00％，与宫内传播组的95．83％、4．17％相比差异有统计学意义（Х^2=5．81，P〈0．05）。单因素分析结果显示FokⅠ位点FF基因型在非宫内传播组的频率为50．00％，显著高于宫内传播组的16．67％（Х^2=5．59，P〈0．05）。TaqⅠ位点TT基因型在宫内传播组的频率为95．80％，显著高于非宫内传播组70．00％（Х^2=5．81，P〈0．05）。结论维生素D受体基因FokⅠ和TaqⅠ多态性可能与乙型肝炎病毒发生宫内传播存在一定的相关性，仍需扩大样本量进一步研究。Objective To investigate association between polymorphisms of vitamin D receptor gene Fok Ⅰ and TaqⅠand intrauterine infection of HBV so as to determine predisposing genes and protecting gens for intruterine transmission of HBV. Methods In simple random sampling method, 24 neonates with and 20 neonates without confirmed intrauterine infection of HBV were selected respectively. The blood samples from formeral vein of all neonates were taken and the DNA in it was extacted. The differences in polymorphism distributions of VDR gene in loci Fok I of exon 2 and in loci Taq Ⅰ of exon 9 of neonates between the two groups were analyzed by using PCR-RFLP method. Results The frequency of VDR-Fok Ⅰ allele f in the neonates in the HBV intrauterine transmission group was 52.08%, which was significantly higher than 27.50% in the non-HBV intrauterine transmission group （Х^2 = 12.36, P 〈 0.05）. The frequency of VDR-Taq Ⅰ allele T in the HBV intrauterine transmission group was 97.92%, which was significantly higher than 85.00% in the non-HBV intrauterine transmission group （Х^2 = 10.87, P 〈 0.05 ）. The frequencies of VDR-Fok Ⅰ genotypes FF, Ff, and ff in the non-HBV intrauterine transmission group were 50.00%, 45.00% and 5.00% respectively, while those in the HBV intrauterine transmission group were 16.67%, 62.50% and 20.83% respectively, there were significant differences between the two groups （Х^2 = 6.72, P 〈 0. 05）. The frequencies of VDR-Taq Ⅰ genotypes TF and Tt in the non-HBV intrauterine transmission group were 70.00% and 30.00% respectively, while those in the HBV intrauterine transmission group were 95.83% and 4.17% respectively, there were significant differences between the two groups （Х^2 =5.81, P 〈0.05）. The univariate analysis showed that the frequency of Fok Ⅰ -FF genotype in neonates in the non- intrauterine infection of HBV was 50.00% , which was higher than 16.67% in the intrauterine infection of HBV group（Х^2 =5.59,P 〈 0.05 ）, and the frequency of Taq Ⅰ -TF g

关 键 词：维生素D受体 基因多态性 乙型肝炎病毒 宫内感染 遗传易感性 

分 类 号：R71[医药卫生—妇产科学] R512.62[医药卫生—临床医学]