棉花T-DNA标签雄性不育突变体的遗传分析  

Genetic Analysis of Cotton Male Sterile Mutant by T-DNA Tagging

作　　者：张玉芹[1,2] 高俊平[2] 张晋[3] 王芙蓉[2] 张军[2] 

机构地区：[1]山东师范大学生命科学学院,山东济南250014 [2]国家棉花改良中心山东分中心/山东棉花研究中心,山东济南250100 [3]山东农业大学生命科学学院,山东泰安271018

出　　处：《山东农业科学》2010年第1期22-25,共4页Shandong Agricultural Sciences

基　　金："十一五"国家科技支撑计划(2006BAD13B04-1-07);国家"863"计划(2006AA10A108);山东省农业良种工程项目(2007LZ005-006);山东省农业科学院自主创新项目(2006YCX011)

摘　　要：利用农杆菌介导T-DNA插入得到棉花雄性不育突变体,与野生型陆地棉(Gossypium hirsutumL.)Coker312杂交得到F1代。F1代植株出现了不育与可育两种性状的分离,分离比是1∶1。对F1代进行形态学观察、卡那霉素和除草剂抗性鉴定、PCR扩增检测以及遗传学分析,证实雄性不育突变性状的表现与T-DNA插入共分离,从而认为突变是由T-DNA插入引起的显性杂合突变,这为利用T-DNA标签法进行雄性不育有关基因的克隆奠定了基础。A male sterile mutant by T - DNA insertion was crossed with a wild type upland cotton （ Cossypium hirsutum L. ） cultivar Coker312. The segregation ratio of male sterile to male fertile was 1： 1 in generation F1. The results of morphological observation, kanamycin and herbicide resistance assay, PCR identification and corresponding genetic analysis showed that the male sterile was co - segregated with the T - DNA insertion. This male sterile mutant was considered as dominant heterozygous mutation caused by the T - DNA insertion, which would lay the basis for cloning the male sterile related genes of upland cotton by T - DNA tagging.

关 键 词：棉花 T—DNA 雄性不育突变体 遗传分析 

分 类 号：S562.035.3[农业科学—作物学]