水稻叶绿素a氧化酶基因突变体的鉴定及其对氮营养的响应  

Identified Mutant of Chlorophyll a Oxygenase in Rice and Its Response to Nitrogen Nutrition

作　　者：李宝珍[1,2] 徐国华[2] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008 [2]南京农业大学资源与环境科学学院,南京210095

出　　处：《生物技术通报》2010年第1期78-82,共5页Biotechnology Bulletin

基　　金：中国博士后科学基金(20070421031);国家重点基础研究发展计划("973"计划)(2005CB120903;2007CB109303)

摘　　要：对从日本获得的水稻Tos17插入突变基因进行了鉴定,并通过PCR技术对其插入位点和纯合体进行了分析和筛选。结果表明,Tos17插入在序列号为DP000086的基因,在此基因反向互补序列的1579bp处,在mRNA序列的第5个外显子区域,是水稻的一个叶绿素a氧化酶基因,而且此基因在单一的铵营养下表达减弱,氮饥饿条件下表达增强。利用Tos17未端和插入位点上下游设计引物进行PCR反应,鉴定到3株纯合突变体株,为进一步研究其功能奠定了基础。A mutant gene of Tos17 insertion was identified in rice,and insertion site and homozygous insertion mutants were identified and screened by the PCR. Results showed that Tos17 was inserted 1579 bp of reverse complement sequence of the gene （ accession No. DP000086 ） , the 5＇h exon in cDNA, which may be a chlorophyll a oxygenase （ CAO ） of rice. Moreover, expression level of the gene was increased by single ammonium nutrition and decreased by nitrogen starvation. Three homozygous insertion mutants were screened using primer based on the sequence of Tos17 3＇end and upstream and downstream of the insertion site of the gene.

关 键 词：水稻 Tos17 叶绿素a氧化酶 纯合体 

分 类 号：S511[农业科学—作物学]