禾谷镰刀菌降解毒素基因Tri101的克隆和序列分析  

Clonging and Sequence Analysis of Tri101 Gene from Fusarium graminearum Schwabe

作　　者：闫海霞[1,2] 杨丽荣[2] 薛保国[2] 段立清[1] 孙虎[2] 全鑫[2] 

机构地区：[1]内蒙古农业大学林学院,呼和浩特010019 [2]河南省农业科学院植物保护研究所,郑州450002

出　　处：《生物技术通报》2010年第1期128-135,共8页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30771439);农业部"948"项目(2008-Z22)

摘　　要：禾谷镰刀菌Tri101基因编码的单端孢酶烯3-O-乙酰转移酶可通过加乙酰基的形式使禾谷镰刀菌产生的单族毒素(如DON)转变为较低的毒性。本研究利用RT-PCR技术从禾谷镰刀菌0623中扩增并克隆了Tri101基因的cDNA片段,测序结果表明,Tri101基因核苷酸序列阅读框架全长1356bp(GenBank序列号:GQ907236),编码451个氨基酸的多肽,推测分子量为49.45kD,等电点为5.14。氨基酸序列同源性比对结果表明,它与Kimura报道的禾谷镰刀菌Tri101氨基酸序列同源性最高,为99.56%,与其它13种镰刀菌的Tri101氨基酸序列的同源性分别为97.91%-75.68%。系统进化树分析结果表明,Fusarium graminearium0623与Fusarium sporotrichioides属于同一进化枝且与Fusarium asiaticum有较近的亲缘关系,而与F.oxysporum、F.moniliforme、F.nygamai、F.nisikadoi和F.decemcellulare的亲缘关系较远。Fusarium graminearum Tril01 encodes a trichothecene 3-O-acetyltransferase that converts DON to a less toxic acetylated form. The cDNA of Tri101 gene was amplied from the Fusarium graminearum 0623 total RNA by RT-PCR. The results showed that Tri101 was 1 356 bp in size and encoded 451 amino acids （GenBank accession number： GQ907236）. The molecular weight and theoretical isoelectric point is 49.45 kD and 5.14, respectively. The deduced amino acid of Fusarium graminearum 0623 Tri101 shared 99.56% homology with the deduced amino acid published by Kimura in GenBank. Alignments with the deduced amino acid of mature proteins in other 13 species of Fusarium showed 97.91% -75.68%. Based on Tri101 amino acid sequence the phylogenic tree of trichothecene 3-O-acetyltransferase gene was established. The results showed that Fg0623 and Fusarium sporotrichioides belong to the same group of organisms, which was closely related to Fusarium asiaticum while distantly related to F. oxysporum, F. moniliforme, F. nygamai, F. nisikadoi and F. decemcellulare.

关 键 词：禾谷镰刀菌 Tri101基因 基因克隆 序列分析 

分 类 号：Q78[生物学—分子生物学] S435.121.4[农业科学—农业昆虫与害虫防治]