从非变性聚丙烯酰胺凝胶回收纯化DNA样本  

Rapid Recovery and Purification of DNA Fragments from Nondenaturing Polyacrylamide Gel

作　　者：朱玉君[1] 樊叶杨[1] 庄杰云[1] 

机构地区：[1]中国水稻研究所国家水稻改良中心水稻生物学国家重点实验室,杭州310006

出　　处：《生物技术通报》2010年第1期193-195,共3页Biotechnology Bulletin

基　　金：国家转基因专项(2008ZX08001-004);水稻生物学国家重点实验室自主研究课题(ZZKT200801)

摘　　要：介绍一种从非变性聚丙烯酰胺凝胶中回收和纯化目标DNA片段的方法,经比较回收纯化前后PCR产物的电泳结果,表明该方法具有简单快捷和效率高的优点。A method of recovering and purifying DNA fragments from nondenaturing polyacrylamide gel was reported. PCR products amplified from the recovered DNA samples were detected by nondenaturing polyacylamide gel electrophoresis and compared with those from the original DNA samples isolated from rice seedlings. Results showed that this rapid method can efficiently recover the target DNA fragments from nondenaturing polyaerylamide gel.

关 键 词：非变性聚丙烯酰胺凝胶 回收纯化 目标DNA片段 

分 类 号：Q78[生物学—分子生物学]