纤维素酶水解老化纸张中纤维素物质的酶活力分析  

Analysis of Cellulase Activity with Hydrolysis Cellulose in Different Aging Paper

作　　者：孟朝阳[1] 

机构地区：[1]辽宁警官高等专科学校公安技术系辽宁省高校物证分析重点实验室

出　　处：《光谱实验室》2010年第1期107-111,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：公安部科技创新项目(2008YYCXLNST021)

摘　　要：应用3,5-二硝基水杨酸测定法,分析了纤维素酶水解老化纸张中纤维素物质的酶活力影响因素,根据酶活力确定了酶解反应条件。pH=4.8,温度为50℃,酶解时间为60min,加酶量为10kU/mL条件下,经纤维素酶水解后测得的还原糖量呈现一定的趋势,可以此来初步判定纸张纤维的老化程度。Analysis of cellulase activity was studied by 3,5-dinitrosalicylic acid （DNS） method. The optimum conditions of enzymolysis were determined as follows：pH4.8,enzymolysis temperature 50℃ ,enzymolysis time 60 min ,and the enzyme solution 10kU/mL. Different aging degree of cellulose paper was determined by the decomposition of reducing sugar measured after a certain amount of trend.

关 键 词：纤维素酶活力 纤维素 纤维老化 

分 类 号：O657.32[理学—分析化学]