核黄素与碘离子相互作用的光谱分析  

Spectral Analysis of the Interaction Between Riboflavin and Iodine Ions

作　　者：张怀斌[1] 李怀祥[2] 郑白玉[1] 黄玉玲[1] 荣先国[1] 

机构地区：[1]滨州医学院烟台校区化学教研室 [2]山东师范大学化学化工与材料科学学院,济南市文化东路250014

出　　处：《光谱实验室》2010年第1期206-209,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：国家自然科学基金(60671010)

摘　　要：研究了核黄素与I-相互作用的荧光光谱和吸收光谱变化,得出I-可以使核黄素的荧光发生有规律的猝灭,它们之间的相互作用属于动态猝灭机制。根据Stern-Volmer方程,求出了动态猝灭常数Ksv=44.72L.mol-1,荧光猝灭速率常数Kq=6.4×1010L.mol-1.s-1。I-对核黄素荧光的这种动态猝灭引起了吸收光谱的变化,吸收峰从373nm蓝移到了369nm,而443nm处吸收峰却红移到了447nm;并且核黄素的吸收强度随I-浓度的增大不断增强,是由于I-重原子效应的影响,使核黄素分子的自旋角动量与轨函角动量强烈的相互作用,S0→S1的吸收跃迁概率增大而造成的。The influence of iodide on the optical property of riboflavin was studied by absorption and fluorescence spectra. The fluorescent emission intensity of riboflavin solution was quenched by iodide. The dominant process for this quenching might be attributed to dynamic quenching. The dynamic quenching constant Ksv and quenching rate constant Kq were 44.72L · mol^-1 and 6.4× 10^10· L · mol^-1 · s^-1 ,respectively,according to the Stern-Volmer equation. The absorption peak position of the riboflavin solution was influenced by iodide and the absorption intensity increased with the iodide concentration,which might be attributed to the increase of transition probability from S0 to S1 due to spin-orbit coupling of the riboflavin molecules.

关 键 词：核黄素 碘离子 荧光猝灭 光谱分析 

分 类 号：O433.4[机械工程—光学工程] O657.32[理学—光学]