杂交相思根瘤菌的抗逆特性  

Rhizobium Tolerance to Temperature,pH Values,NaCl Concentration Stresses on Acacia mangium×A. auriculiformis

作　　者：李莉[1] 吕成群[1] 伍荣善 黄宝灵[1] 吴庆梅 秦慧晶 

机构地区：[1]广西大学林学院,南宁530004 [2]广西国营钦廉林场 [3]广西藤县林业局 [4]广西融安县林业局

出　　处：《林业科技开发》2010年第1期32-35,共4页China Forestry Science and Technology

基　　金：广西科学基金(编号:桂科基0448005);广西"十五"林业科学研究项目(编号:林科字2002-19)

摘　　要：对20个分离自广西不同地方的杂交相思根瘤菌菌株进行温度、pH值、NaCl及抗生素的抗逆性研究。结果表明，65％的菌株能在39℃时生长良好；100％的菌株能在pH值5．0—9．0的条件下生长良好；所有菌株在2．0％浓度NaCl下均不能生长，有5个菌株在1．5％浓度下能生长。菌株对不同抗生素表现出不同的抗性，有16个菌株对Tc有抗性，仅有2个菌株对Km有抗性。不同的菌株对抗生素表现出不同的抗性，Z’2-7抗性最强，而L1-6、12—7、L3—2、S＇3--6抗性最差。Twenty strains of Acacia rhizobium- isolated from A. mangium × A. auriculiformis plantation grown in the different areas in Guangxi Province were examined for tolerance to the stresses of temperature, pH, NaCl and intrinsic antibiotics resistance. The results showed that 65% of strains tested grew well at 39 ℃ and 20 strains grew well at the range of pH 5.0 - 9.0. All strains could not grow in the media contained 2.0% NaCl, but five strains could grow in the media contained 1.5% NaCl. For the intrinsic antibiotics resistance, sixteen strains of 20 were resistant to Tc, however only two strains were resistant to Km. The strain Z＇2-7 was the greatest in resistance to intrinsic antibiotics, while the strain L1-6, L2-7, L3-2 and S＇3-6 were the lowest.

关 键 词：杂交相思 根瘤菌 温度 耐盐性 pH值耐受性 抗生素抗逆性 

分 类 号：S792.99[农业科学—林木遗传育种] Q939.114[农业科学—林学]