两种马铃薯块茎总RNA提取方法的比较  

Comparative study on two methods of total RNA extraction from Potato Tubers

作　　者：赵宝勰[1,2] 程李香[1,3] 林必博[1] 王蒂[1,2] 王玉萍[1] 

机构地区：[1]甘肃省作物遗传改良与种质创新重点实验室,甘肃兰州730070 [2]甘肃农业大学农学院,甘肃兰州730070 [3]甘肃农业大学研究测试中心,甘肃兰州730070

出　　处：《广东农业科学》2010年第1期124-127,共4页Guangdong Agricultural Sciences

基　　金：教育部新世纪优秀人才支持计划(MCET-07-0214);国家自然科学基金(30960205);甘肃省自然科学基金(0710RJZA095)

摘　　要：以马铃薯普通栽培品种"大西洋"的试管薯为材料,针对马铃薯块茎富含多糖和酚类化合物的特点,分别采用Chan法和Trizol法提取马铃薯块茎总RNA。结果表明,Chan法比Trizol法更适宜提取马铃薯块茎总RNA,该法提取的总RNA的28S、18S和5S rRNA条带清晰可见,无降解现象,未被蛋白质、多糖、酚类物质以及残余试剂所污染,且通过RT-PCR技术可以成功扩增出马铃薯腺苷二磷酸葡萄糖焦磷酸化酶AGPase小亚基基因。Potato tubers were rich in polysaccharides and phenolic compounds. To approach an effective method for total RNA extraction of tubers, two methods （Chan method and Trizol method） were used to extract the total RNA from tubers. The results indicated that Chan method was more suitable than Trizol method in the extraction of tuber total RNA. The total RNA extracted by this method showed clear bands of 28S, 18S and 5S rRNA, and no degradation. There were also＇ no pollution of protein, polysaceharides, phenolic compounds and residual reagents. The potato AGPase small subunit gene could be amplificated successfully with tile use of total RNA extracted by Chan method as template.

关 键 词：马铃薯块茎 总RNA提取 Chan法 TRIZOL法 

分 类 号：S532[农业科学—作物学]