藏獒RAPD-PCR反应体系的优化  

Optimization of RAPD-PCR Reaction System for Tibetan Mastiff

作　　者：兰小平[1] 李三相[1] 李一婧[1] 王廷璞[1] 黄晶[1] 鄢珣[2] 崔泰保[2] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741000 [2]甘肃农业大学

出　　处：《畜牧兽医杂志》2010年第1期1-3,共3页Journal of Animal Science and Veterinary Medicine

摘　　要：以藏獒血液为试验材料,提取基因组DNA后,对藏獒RAPD反应体系进行了优化。结果表明,20μL的最佳反应体系为:2.5 mmol/LMg2+、0.6 mmol/L dNTP、50 ng模板DNA、1.25UTaq酶和0.3μmol/L引物;藏獒RAPD反应的最佳退火温度为36.5℃。The RAPD -PCR reaction system was optimized after the genomic DNA was extracted from the blood of Tibetan Mastiff. The results showed that a total volume of 20 μL optimal RAPD - PCR reaction system of Tibetan Mastiff consisted of 2.5 mmol/L Mg2＋ ,0.6mmol/LdNTP,50ng genomic DNA, 1.25U Taq DNA polymerase , and 0.3 μmol;L primers. The best renaturation temperature of RAPD analysis of Tibetan Mastiff was 36.5℃.

关 键 词：藏獒 RAPD—PCR 体系优化 

分 类 号：S829.2[农业科学—畜牧学]