阳离子树脂分离纯化两面针中总生物碱的工艺研究  

Study on separation and purification process of total alkaloids from Zanthoxylum nitidum by cation exchange resin

作　　者：卢凌春[1] 方琳乔[1] 龙盛京[1] 

机构地区：[1]广西医科大学药学院,广西南宁530021

出　　处：《西北药学杂志》2010年第1期17-19,共3页Northwest Pharmaceutical Journal

基　　金：广西自然科学基金资助项目(桂科自0832158)

摘　　要：目的研究用阳离子树脂富集纯化两面针中总生物碱的工艺条件。方法以总生物碱的吸附量和解吸率为考察指标,采用UV分光光度法测定两面针中总生物碱的含量,对4种不同型号的阳离子树脂进行评价,并考察最佳工艺和参数条件。结果每g Ls006阳离子树脂对两面针总生物碱最大吸附量为32.90 mg,解吸率为94.78%,总生物碱保留率为47.17%,结晶产品以氯化两面针碱计含量达89.07%,得率大于50%。结论采用该法可以分离得到纯度较高的总生物碱。Objective In order to obtain the optimal conditions for separating the alkaloids from the extract of Zanthoxylurn nitidum by selecting appropriate cation exchange resins. Method Four types of cation exchange resin were evaluated in separating efficiency with measuring the adsorption ratio and eluting ratio of total alkaloids as indexes, and the content of total alkaloids from Zanthoxylurn nitidurn was determined as an index by spectrophotometry to choose optimal technological parameters. Result The Ls006 cation exchange resin showed the best separating efficiency. Each gram of Ls006 cation exchange resin could absorb 32.90 mg of the total alkaloids, and the desorption ratio was 94.78 %. After enrichment and purification, the yielding rate of the alkaloids was 47.17% ,and the content of nitidine chloride in the crystamzation from mother liquor was 89. 07% ,the yield of Nitidine chloride was over 50%. Conclusion The Ls006 cation exchange resin can be used for purificating total alkaloids from Radix of Zanthoxylum nitidum ,and the established procedure is simple and feasible.

关 键 词：两面针 总生物碱 阳离子树脂 纯化 分光光度法 

分 类 号：R284.2[医药卫生—中药学]