早期变应原免疫对小鼠后期变应性鼻炎影响的研究  

Effect of early allergen immunization on the development of allergic rhinitis in mouse

作　　者：周玥[1] 陈建军[1] 鲁忠元[1] 孔维佳[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430030

出　　处：《中华耳鼻咽喉头颈外科杂志》2010年第1期52-55,共4页Chinese Journal of Otorhinolaryngology Head and Neck Surgery

摘　　要：目的探讨新生小鼠早期变应原免疫对后期变应性鼻炎（allergic rhinitis，AR）发生发展的影响。方法BALB／c新生小鼠24只，按随机数字表法分为4组：小剂量组、大剂量组、阴性对照组、阳性对照组，每组6只。于出生后第1、5、12天小剂量组皮下注射0．2mg／ml的卵清蛋白（ovalbumin，OVA）生理盐水溶液0．05ml，大剂量组皮下注射20mg／ml的OVA生理盐水溶液0．05ml，阴性对照组和阳性对照组皮下注射同等剂量生理盐水。待小鼠长至6周时，开始对小剂量组、大剂量组、阳性对照组以OVA生理盐水腹腔注射和滴鼻诱发AR，阴性对照组予同等剂量的生理盐水腹腔注射和滴鼻诱发AR。观察小鼠症状、鼻黏膜组织学改变、检测血清OVA—IgE水平以及脾细胞培养上清液中白细胞介素（interleukin，IL）4、IL-5和γ干扰素（interferon，IFN）水平的差异。结果新生小鼠皮下注射大剂量变应原组后期所诱发的AR与阳性对照组相比，症状明显减轻，鼻黏膜变态反应性炎症明显减轻。大剂量组血清OVA—IgE、IL-4、IL-5水平（x±s，以下同）分别为（265．11±26．29）、（446．39±72．83）、（171．24±15．66）pg／ml，分别低于阳性对照组的（665．85±43．15）、（1113．45±30．47）、（255．36±30．96）pg／ml，差异有统计学意义（t值分别为0．000、0．000、0．009，P值均〈0．05），而IFN-γ水平为（319．74±56．30）pg／ml，显著高于阳性对照组的（170．02±14．50）pg／ml，差异有统计学意义（t=0．000，P〈0．05）；而皮下注射小剂量变应原组的各指标改变与阳性对照组比较差异无统计学意义。结论新生小鼠早期大剂量OVA皮下注射可对其后期由同种变应原所诱发的AR产生保护作用。Objective To investigate the effect of early allergen exposure on later development of allergic rhinitis in mouse. Methods Twenty-four BALB/e neonates were randomly divided into 4 groups （low-dose group, high-dose group, negative control group and positive control group） , each group had 6 mice. The mice were administered ovalbumin （OVA） by subcutaneous injection on day 1 , 5, 12 after birth （ 10 μg OVA in 0.05 ml saline for low-dose group, 1000 μg OVA in 0.05 ml saline for high-dose group, only saline for negative and positive control group ）. Then the mice were sensitized and intranasally challenged with OVA （saline without OVA was used in negative control group） after 6 weeks. Symptoms, histopathological changes of nasal mucosa were observed, OVA-IgE in serum was examined, cytokines IL-4, IL-5 and IFN-γwere detected in the supernatant of cultured splenic mononuclear cells. Results Compared to the positive control group, symptoms and nasal mucosa histological changes of high-dose group was indistinctive. The level of OVA-IgE and cytokines IL-4, IL-5 （x ± s） in high-dose group [ （265. 11 ± 26. 29）, （446.39 ± 72.83） and （171.24 ± 15.66） pg/ml, respectively] were significantly lower than those in positive control group [ （665.85 ± 43. 15 ）, （ 1113.45 ± 30.47 ）, （255.36 ± 30.96） pg/ml, respectively, t value were 0. 000, 0. 000 and 0. 009, respectively, all P 〈 0. 051. The level of IFN-3, in high-dose group [ （319.74 ± 56.30） pg/ml] was significantly higher than those in positive control group [ （170.02 ± 14. 50） pg/ml, t = 0. 000, P 〈 0.05 ]. There was no significant difference of the results between the low-dose group and positive control group. Conclusions Neonatal immunization with high-dose OVA inhibited the future allergic rhinitis symptoms, nasal histological changes, serum OVA-IgE levels and Th1/Th2 cytokine imbalance, resulting in the protective effect.

关 键 词：变应原 小鼠 鼻炎 变应性 常年性 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]