桂西地区克拉霉素耐药的Hp基因分型研究  

The genotyping of clarithromycin-resistant Hp isolates from patients in the west of Guangxi

作　　者：黄衍强[1] 黄宏思[1] 黄赞松[1] 秦静英[1] 黄小凤[1] 周喜汉[2] 岑朝[2] 

机构地区：[1]右江民族医学院,百色533000 [2]右江民族医学院附属医院

出　　处：《中华检验医学杂志》2010年第1期37-41,共5页Chinese Journal of Laboratory Medicine

摘　　要：目的研究桂西地区对克拉霉素耐药的Hp基因分型。方法于2007年3—10月在右江民族医学院附属医院胃镜室采集就诊患者胃黏膜标本，共采集患有胃炎、消化性溃疡的患者胃黏膜标本247份，分离培养获得126株Hp，E-test进行药敏实验，REP—PCR方法进行基因分型，并运用NTsys_2软件进行聚类分析，同时收集携带耐药菌株病例的临床资料。结果桂西地区对克拉霉素耐药的26株Hp按照相似性78％被分成6类基因型，分别是Ⅰ～Ⅵ类，每类分别有2、11、1、8、3、1株耐药性Hp；其中Ⅱ类菌株均来自胃溃疡，而且大多数来自壮族患者；Ⅳ类菌株均来自胃炎。结论REP—PCR可以把对克拉霉素耐药的Hp分成6大类基因型，Hp感染患者的疾病类型、民族、家族胃病史等可能与基因型有关。Objective To study the genotyping of clarithromycin-resistant Helicobacter pylori （Hp） isolates. Methods From March 2007 to October 2007,247 gastric mucosa specimens were collected by the endoscopy from the patients with peptic ulcer or gastritis at the affiliated hospital of Youjiang Medical College for Nationalities. A total of 126 Hp strains were isolated. Resistance to clarithromycin in Hp was determined by E-test. All of the resistant isolates were genotyped with REP-PCR and further clustered with NTsys 2 software. The clinical data was collected for these patients with clarithromycin-resistant Hp. Results The 26 clarithromycin-resistant isolates from the west of Guangxi were divided into six genotypes including Group Ⅰ , Group Ⅱ, Group Ⅲ, Group Ⅲ, Group Ⅳ, Group Ⅴ and Group Ⅵ according to the homology of 78%. Every group have 2, 11, 1, 8, 3, 1 strains Hp, respectively. Strains of group 11 were isolated from patients with peptic ulcer and most of them from Chuang patients. All strains of group IV were isolated from patients with gastritis. Conclusions The clarithromycin-resistant isolates were divided into six groups by REP-PCR. Disease type, nationality of patients and family history of stomach diseases were associated with the genotypes.

关 键 词：螺杆菌 幽门 克拉霉素 抗药性 细菌 基因型 聚合酶链反应 

分 类 号：R446.5[医药卫生—诊断学]