七带石斑鱼染色体核型研究  

Chromosome karyotype of sevenband grouper Epinephelus septemfasciatus (Thunberg,1793)

作　　者：钟声平[1,2] 陈超[2] 王军[1] 刘志鸿[2] 柳淑芳[2] 朱玲[2] 庄志猛[2] 

机构地区：[1]厦门大学海洋环境学院,福建厦门361005 [2]山东省渔业资源与生态环境重点实验室,中国水产科学研究院黄海水产研究所,山东青岛266071

出　　处：《中国水产科学》2010年第1期150-155,共6页Journal of Fishery Sciences of China

基　　金：青岛市科技发展计划项目(08-2-3-6-hz);农业部948项目(2008-Z8)

摘　　要：采用PHA体内直接注射法制备了七带石斑鱼(Epinephelus septemfasciatus)头肾组织染色体标本并分析其核型。结果表明,七带石斑鱼二倍体染色体数为48,24对染色体均为端部着丝粒染色体,其染色体臂数(NF)为48,核型公式为:2n=48t,NF=48。未发现与性别相关的异型染色体,第24对染色体长度明显小于其他染色体。通过与其他22种石斑鱼染色体核型进行比较,发现七带石斑鱼具有石斑鱼属鱼类的原始核型特征,属于石斑鱼属鱼类的原始类群。The metaphase chromosomes of Epinephelus septemfasciatus were obtained from head kidney tissue by the method of PHA and colchicine injection and the karyotype was analyzed. The results showed that there were 48 telocentric chromosomes in the diploid and their karyotypic formula was 2n=48t, NF=48. The length of the 24th chromosome was distinctively shorter than that of others, while there was no obvious difference in length among the other 23 chromosomes. By comparison with the chromosome karyotypes of other 22 Epinephelus species, it can be concluded E. septemfasciatus has the representative characteristics of ancestral karyotypic pattern and belongs to the primitive grouper.

关 键 词：七带石斑鱼 染色体 核型 

分 类 号：S91[农业科学—水产科学]