基于线粒体D-loop区与COI基因序列比较分析养殖与野生银鲳群体遗传多样性  

Comparative analysis on the genetic diversity of cultured and wild silver pomfret populations based on mtD-loop and COI gene

作　　者：彭士明[1] 施兆鸿[1] 侯俊利[1] 

机构地区：[1]中国水产科学研究院东海水产研究所,上海200090

出　　处：《水产学报》2010年第1期19-25,共7页Journal of Fisheries of China

基　　金：中央级公益性科研院所基本科研业务费(东2008M14;东2007Z02);沪农科攻字(2007)第4-1号

摘　　要：运用线粒体D-loop区与COI基因片段序列比较分析了养殖与野生银鲳群体的遗传多样性。研究结果显示,线粒体D-loop区片段中,A、T、C与G4种核苷酸的平均含量分别为40.00%、30.55%、16.75%和12.70%,A+T的含量为70.55%,明显高于G+C的含量。COI基因片段中,A、T、C和G的平均含量分别为25.85%、33.90%、21.30%和18.85%,A+T的含量(59.75%)同样高于G+C的含量。基于D-loop序列分析所得出的两群体总的变异位点、单倍型数、单倍型多样性、核苷酸多样性及平均核苷酸差异数分别为19、15、0.895、0.007和2.505。基于COI基因所得出的两群体总的变异位点、单倍型数、单倍型多样性、核苷酸多样性及平均核苷酸差异数分别为33、17、0.713、0.004和2.239。基于线粒体D-loop区与COI基因片段序列的研究结果均显示,养殖银鲳群体的遗传多样性低于野生群体的遗传多样性。养殖群体基于线粒体D-loop区与COI基因片段分析得出的单倍型多样性分别为0.562与0.571,野生群体基于线粒体D-loop区与COI基因片段分析得出的单倍型多样性分别为0.891与0.801。基于D-loop序列的分子方差(AMOVA)分析结果显示,养殖与野生银鲳群体间具有较高的遗传分化,而基于COI基因片段AMOVA分析结果显示,两群体间并无明显的遗传分化。研究表明,线粒体D-loop区与COI基因均可作为检测银鲳群体遗传多样性的有效标记,但线粒体D-loop区作为反映银鲳群体间遗传多样的敏感度要高于COI基因。The genetic diversity of cultured and wild silver pomfret （Parnpus argenteus） populations was analyzed based on mtD-loop and CO1 gene. The results showed that the average A, T, C and G contents in D-loop were 40.00%, 30.55%, 16.75% and 12.70%, respectively, and the contents of A ＋ T were 70.55%, higher than those of G ＋ C. In the COl gene, the average A, T, C and G contents in D-loop gene were 25.85%, 33.90%, 21.30% and 18.85%, respectively, and the contents of A ＋ T were 59.75%, also higher than those of G ＋ C. The total variable sites, number of haplotypes （h）, haplotype diversity （Hd）, nucleotide diversity （π） and mean pairwise nucleotide differences （k） of two populations based on D-loop were 19, 15, 0. 895, 0. 007 and 2. 505, respectively. The same parameters based on COI gene were 33, 17, 0. 713, 0. 004 and 2. 239, respectively. Based on mtD-loop and COl gene, the genetic diversity of cultured population was lower than that of wild population. The Hd of cultured population based on mtD-loop and CO1 gene were 0.562 and 0. 571, respectively. And the lid of wild population were 0.891 and 0. 801, respectively. The AMOVA analysis based on D-loop showed that the significant genetic divergence existed in cultured and wild populations, while there was no significant genetic divergence when it was analyzed based on CO1 gene. In conclusion, both D-loop and CO1 gene were effective molecular markers for analyzing the genetic diversity of silver pomfret population, while the sensitivity of D-loop in detecting the genetic diversity among populations was higher than that of CO1 gene.

关 键 词：银鲳 线粒体DNA 养殖 野生 遗传多样性 

分 类 号：Q346[生物学—遗传学] S917[农业科学—水产科学]