合浦珠母贝双列杂交家系的建立与遗传分析  

Establishment and genetic analysis of complete diallel cross families of pearl oyster(Pinctada fucata)

作　　者：谷龙春[1,2] 李金碧[1,3] 喻达辉[1] 黄桂菊[1] 刘建业[1,2] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部海水养殖生态与质量控制重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]华中农业大学水产学院,湖北武汉430070

出　　处：《水产学报》2010年第1期26-31,共6页Journal of Fisheries of China

基　　金：国家科技支撑计划(2006BAD01A13);国家"八六三"高技术研究发展计划(2006AA10A409);广东省科技计划(2006B20201063)

摘　　要：以采自广西北海(B)、广东徐闻(X)、海南三亚(S)的3个不同养殖群体的合浦珠母贝为亲贝,按照完全双列杂交的方式,建立了9个交配组合,每个组合10个家系,共90个家系。出苗经60d养殖后,比较各家系的育种值,并对家系进行综合评价。结果表明:壳长的Kung育种值大于20的家系有8个,分别为BX2(表示北海♂×徐闻♀的2号家系,余依此类推)、BX9、BB4、BB5、BB8、BS9、XS3、SB8,其中XS3的壳长及壳宽的Kung育种值和综合评定值均最大,XX3最小。BB4和XS3的综合评定值大于1。壳长的一般配合力为三亚群体17.40,徐闻17.45,北海18.27;壳高为三亚14.74,徐闻14.79,北海15.54。壳长的特殊配合力为三亚—徐闻17.75,三亚—北海18.27,徐闻—北海18.12;壳高为三亚—徐闻14.98,三亚—北海15.51,徐闻—北海15.46。壳长的平均杂种优势为三亚—徐闻1.41,三亚—北海0.96,徐闻—北海0.67;壳高为三亚—徐闻1.13,三亚—北海1.60,徐闻—北海1.19。表明北海的亲本较好。In order to build base populations for selective breeding, ninety families in nine groups were constructed using a full diallel cross design with parents of Pinctada fucata from three different places I Beihai （B） , Xuwen （X） and Sanya （ S ） ]. Comparisons of the breeding value and general evaluation among families were studied after 60 days culture in out-door pond. The resuks showed that the Kung breeding values of shell length were over 20 in eight families （BX2, Bxg, BB4, BB5, BBS, BS9, XS3 and SB8 ）. The family XS3 had the largest breeding values in shell length and shell width and comprehensive value, whereas XX3 had the smallest ones. The overall values of BB4 and XS3 were over one. General combining abilities （GCA） in shell length for Sanya, Xuwen and Beihai parents were 17.40, 17.45 and 18.27, respectively while GCAs in shell height were 14.74, 14.79 and 15.54, respectively. Specific combining abilities （SCA） in shell length were 17.75, 18.27 and 18.12 for Sanya（ ♂ ） -Xuwen （ ♀ ）, Sanya-Beihai and Xuwen-Beihai matings, respectively while SCAs in shell height were 14. 98, 15.51 and 15.46, respectively. Average heteroses in shell length were 1.4], 0.96 and 0.67 for Sanya- Xuwen, Sanya-Beihai and Xuwen-Beihai matings, respectively while average heteroses in shell height were 1.13, 1.60 and 1.19, respectively. This study suggested that the parents from Beihai are preferable.

关 键 词：合浦珠母贝 遗传育种 双列杂交 家系 

分 类 号：Q31[生物学—遗传学] S917[农业科学—水产科学]