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作 者:张涛[1] 颜世伟[1,2] 庄平[1,2] 章龙珍[1] 田美平[1] 杨金海[1]
机构地区:[1]中国水产科学研究院东海水产研究所,农业部海洋与河口渔业重点开放实验室,上海200090 [2]大连水产学院生命科学与技术学院,辽宁大连116023
出 处:《水产学报》2010年第1期72-79,共8页Journal of Fisheries of China
基 金:农业科技成果转化资金项目(2008GB23260408);国家"八六三"高技术研究发展计划(2008AA10Z227);中央级公益性科研院所基本科研业务费专项资金(2007M01);浙江省科技计划项目(2007C12026);上海教育委员会E-研究院建设资助项目(E03009)
摘 要:利用超声波检查仪对人工养殖西伯利亚鲟的性别及性腺发育进行超声波扫描,并利用微创手术检查和组织学观察法来验证超声波对西伯利亚鲟性别及发育时期扫描的鉴定结果。通过对比研究表明:超声波方法对西伯利亚鲟性别鉴定的准确率较高,对2~5龄雌性鉴定的准确率为95%,对雄性鉴定的准确率为87%。超声波技术鉴定性腺发育的准确率随性腺的发育而提高,鉴定早期性腺(Ⅰ~Ⅱ期)发育的准确率(65.25%)低于对晚期性腺(Ⅲ~Ⅴ期)发育鉴定的准确率(88.65%)。超声波技术鉴定西伯利亚鲟性别及性腺发育速度较快,根据鱼的发育状况,平均每尾鱼所用的时间约30s。The sex and gonad development in cultured Acipenser baerii was scanned by ultrasonic scanning. The results of sex and development stages of scanned A. baerii were identified by Minimally Invasive Surgical technique and histological examination. By comparative study, the results showed that there was higher accuracy of sex identification in A. baerii by ultrasonic method, the accuracy of identification in female was 95% higher than that in male, which was 87%. Success of gonad development identification by ultrasonic method was decided by gonad development, the accuracy of gonad development in early stages ( stages Ⅰ - Ⅱ ) Was 65.25% lower than that in later stages ( stages Ⅲ - Ⅴ ), which was 83.65%. The speed of sex identification using ultrasonic technology was faster, according to the developmental status of A. baerii, the duration time was only 30 s.
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