干燥综合征患者外周血CD5^+ B细胞、CD5 mRNA变化的研究  

Changes of CD5^+ B Cells and CD5 mRNA in Peripheral Blood of Sjgren's Syndrome Patients

作　　者：肖睿璟[1,2] 李莉[1,2] 刘婧依[1,2] 李杰[1,2] 陈勇[3] 谭锦泉[1,2] 边专[3] 何玉玲[1] 

机构地区：[1]武汉大学医学院医学结构生物学研究中心,湖北武汉430071 [2]武汉大学医学院免疫系,湖北武汉430071 [3]武汉大学口腔医学院,湖北武汉430079

出　　处：《武汉大学学报（医学版）》2010年第1期13-15,42,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金计划创新群体项目(编号:2007ABC010);武汉大学2008年博士研究生(含1+4)自主科研资助项目(编号:20083010201000071)

摘　　要：目的:了解干燥综合征患者外周血中CD5+B细胞的变化及其B淋巴细胞CD5在基因水平上的表达,探讨CD5+B细胞与干燥综合征的关系。方法:采用免疫荧光双标记技术和流式细胞仪检测了20例干燥综合征患者和10例健康对照者外周血中CD5+B细胞(CD5+CD19+)的百分率。应用流式细胞仪分选B淋巴细胞的基础上,运用Real-time RT-PCR方法检测7例干燥综合征患者外周血CD5 mRNA的表达水平。结果:干燥综合征患者外周血CD5+B(CD5+CD19+)细胞的百分率高于健康对照组(P<0.05)。86%的患者CD5 mRNA高于正常人水平。结论:干燥综合征患者外周血CD5+B细胞及CDS转录水平和干燥综合征的发病有一定的关联。To explore CD5^＋ B-lymphocyte cells changes and CD5 gene expression in periph-eral blood of Sjǒgren＇s syndrome patient, and furthermore, to investigate the relationship between CD5^＋ B cells and pathogenesis of Sjǒgren＇s syndrome. Methods： The percentage of CD5^＋ B cell （CD5^＋CD19^＋） in 20 patients with Sjǒgren＇s syndrome and 10 healthy controls was detec ted using immunofluorescence double staining technique and flow cytometry. The real-time RTPCR method was adopted to detect CD5 mRNA expression in 7 patients with Sjǒgren＇s syndrome. Results： The percentage of CD5^＋ B cells in the patients with Sjǒgren＇s syndrome was increased as compared with that in the healthy controls （P〈0.05）. The level of CD5 mRNA in 86% patients was higher than that in the healthy controls. Conclusion： CD5^＋ B cell has relation with the pathogenesis of Sjǒgren＇s syndrome at the cellular level and genetic level.

关 键 词：于燥综合征 CD5^+B细胞 流式细胞术 

分 类 号：R442.8[医药卫生—诊断学]