硫糖脂Sulfatide对受损血管内皮局部炎症细胞黏附的促进作用  

Sulfatide Increases Polymorphonuclear Leukocyte Adhesion to the Injured Endothelium

作　　者：程新耀[1] 陈为民[2] 张维[1] 何精选[1] 金雅磊[3] 王桦[3] 

机构地区：[1]武汉大学中南医院心血管超声检查室,湖北武汉430071 [2]武汉大学中南医院医学动物实验中心,湖北武汉430071 [3]武汉大学中南医院老年病科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2010年第1期26-28,F0002,共4页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金项目(编号:2007ABA074);教育部归国人员启动基金项目

摘　　要：目的:探讨硫糖脂sulfatide在动脉血管内皮损伤早期阶段的作用。方法:将新西兰大白兔20只随机分成两组,左侧颈总动脉用动脉夹夹闭10 min,造成局部血管内膜短暂缺血损伤,再用血管钳临时封闭损伤部位两端,放开动脉夹后实验组予以sulfatide(5 g/L,2 000 ng/g)局部灌注30 min,再通血流,回养36 h后处死动物,做HE染色组织切片检查。对照组以不含sulfatide的PBS溶剂为对照。结果:实验组血管内膜表面黏附的炎症细胞数量较对照组明显增多,右侧未损伤血管内膜无炎症细胞黏附。结论:Sulfatide促进炎症细胞在血管损伤内膜表面黏附聚集,可能在动脉粥样硬化发生过程中具有促进损伤的作用。Objective： To investigate the role of sulfatide in the injury and repair process of rabbit arterial endothelia at the early stage. Methods： Twenty New Zealand rabbit models of arterial endothelial ischemia/reperfusion were established by clamping left common carotid for 10 minutes, and then were divided into sulfatide （5 g/L,2 000 ng/g） treatment group and control group, with 10 rabbits in each group. Pathological examinations of the common carotid were performed after the rabbits were sacrificed. Results： The polymorphonuclear leukocytes adhered to the injured endothelium of sulfatide group were more than those of control group. Conclusion： Sulfatides increased the polymorphonuclear leukocyte adhesion to the site of injured endothelium.

关 键 词：硫糖脂 动脉粥样硬化 血管内皮损伤 

分 类 号：R543.5[医药卫生—心血管疾病]