改良型非创伤性股骨头缺血坏死动物模型的建立  

Establishment of a Modified Model of Non-Traumatic Avascular Necrosis of Femoral Head in Rabbits

作　　者：崔西龙[1] 李章华[1] 戴双武[1] 李聃[1] 杜立[1] 奚美丽[1] 岳朝宏[1] 陈友浩[1] 

机构地区：[1]武汉大学人民医院骨一科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2010年第1期61-64,110,I0002,共6页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30700854)

摘　　要：目的:探讨改良液氮冷冻法制作非创伤性股骨头缺血坏死(ANFH)的可行性,寻找一种较为理想的ANFH的造模方法。方法:将20只新西兰大白兔利用改良液氮冷冻法,制作ANFH模型,分别在术后2,4,6,8周行大体观、MRI、X线及Masson三色染色观察。结果:造模后2周股骨头变化不明显;造模后4周股骨头表面软骨变性,软骨下骨部分坏死;造模后6周股骨头表面软骨部分剥脱,软骨下骨坏死增多;造模后8周股骨头大部分骨质坏死,甚至出现塌陷。结论:改良液氮冷冻方法制作的股骨头坏死模型,其影像学及组织学改变较为符合早中期股骨头坏死的改变,且此法制作模型简单,费用低,坏死表现早,成功率高。Objective： To explore the feasibility of developing non-traumatic avascular necrosis of femoral head （ANFH） by improved liquid nitrogen freezing method, and to find a better way to establish the model of ANFH. Methods： Twenty New Zealand white rabbit models of ANFH were established by improved liquid nitrogen frozen method. The rabbits were examined through gross observation, MRI, X-ray film, and Masson staining at the end of the 2nd, 4th, 6th and 8th weeks post operation respectively. Results： Models of the femoral head had no significant changes during the first two weeks post-operation; the surface cartilage of the femoral head degenerated and the subchondral bone showed necrosis partly after four weeks; the surface femoral head cartilage was partly stripped and the subehondral bone necrosis was severe after six weeks; the majority of femoral head became necrosis and the subchondral bone was even collapsed after eight weeks. Conclusion： The rabbit models of ANFH are established successfully by modified method of liquid nitrogen freezing, the images and histological changes are more consistent with the early and mid-term changes in femoral head. The method is simple and inexpensive, the necrosis appears early and the successful rate is high.

关 键 词：股骨头缺血坏死 造模 液氮冷冻法 

分 类 号：R683[医药卫生—骨科学]