金葡菌对高糖状态下THP-1人单核细胞表达iNOS和IL-1β的影响  

The Effect of Staphylococcus Aureus on Expression of iNOS and IL-1β in THP-1 Monocytes under High Glucose Concentration

作　　者：陈樱[1,2] 孟东[1] 孙培[1] 王宝利[1] 邓为民[2] 姚智[2] 

机构地区：[1]天津医科大学代谢病医院,300070 [2]天津医科大学免疫教研室

出　　处：《天津医药》2010年第1期11-13,共3页Tianjin Medical Journal

基　　金：天津医科大学科学基金资助项目(项目编号:2006KY23)

摘　　要：目的:探讨高糖状态下金葡菌对THP-1人单核细胞表达iNOS和IL-1β的影响。方法:体外培养THP-1细胞,采用2×2析因分析实验设计,以高糖、细菌2个因素,每个因素2个水平交叉分为对照组、高糖组、细菌组和高糖细菌组。各组提取细胞总RNA,应用半定量逆转录聚合酶链反应(SQ-RT-PCR)法,检测诱导型一氧化氮合酶(iNOS)和白细胞介素(IL)-1β的表达情况。结果:高糖和细菌及两者交互效应均影响THP-1人单核细胞表达iNOS和IL-1β。高糖组与对照组比较细胞表达iNOS和IL-1β均减弱(P<0.01);高糖细菌组表达iNOS和IL-1β均显著低于细菌组(P<0.01)。细菌组与对照组比较iNOS和IL-1β表达均增加;高糖细菌组与高糖组比较IL-1β表达增加,iNOS表达变化不显著。结论:高糖状态下,单核细胞受到细菌感染时表达iNOS和IL-1β功能减弱,提示糖尿病患者抗感染能力降低与高血糖有关。Objective：To investigate the effect of staphylococcus aureus on expressions of inducible nitric oxide synthase （iNOS） and interleukin-1 beta （IL-1β） in THP-1 monocytes under high glucose concentrations. Methods：THP-1 monocytes were incubated at different surroundings with 2×2 factorial design. There were 4 experimental groups in the study,which were analyzed by univariate analysis of variance. The total RNA was distilled. The expressions of iNOS and IL-1β were examined by SQ-RT-PCR. Results：The expressions of iNOS and IL-1β were affected by high glucose concentration,bacteria and both of them together in THP-1 monocytes. The expressions of iNOS and IL-1β were weakened in high glucose concentration compared with that of control （P〈0.01）. The expressions of iNOS and IL-1β were weakened in the high glucose concentration and bacterial infection compared with that of the high glucose concentration only（P〈0.01）. The expressions of iNOS and IL-1β increased in bacterial infection compared with that of control （P〈0.01）. The expression of IL-1β increased in high glucose concentration and bacterial infection compared with that of the high glucose concentration only（P〈0.01） but the expression of iNOS was not distinct. Conclusion：When staphylococcus aureus infected monocytes,the expressions of iNOS and IL-1β were weakened in high glucose concentration,which suggested that the depressed function of immunocyte was related with high glucose concentration.

关 键 词：单核细胞 葡萄球菌 金黄色 感染 葡萄糖 一氧化氮合酶 白细胞介素1Β 

分 类 号：R392.11[医药卫生—免疫学] R742.1[医药卫生—基础医学]