依那普利降低老龄鼠心房颤动诱发率的研究  

Enalapril Decreased Inducible Atrial Fibrillation in Old Rats

作　　者：刘英明[1] 杨晔[1] 费宇行[1] 曹毅[1] 卢才义[2] 

机构地区：[1]海军总医院心内科,北京100037 [2]解放军总医院老年心血管病研究所

出　　处：《天津医药》2010年第1期49-51,共3页Tianjin Medical Journal

摘　　要：目的:研究依那普利对老龄鼠心房颤动(房颤)诱发率的影响。方法:雄性老龄Wistar大鼠随机分为对照组和实验组,对照组常规饲养,实验组加喂依那普利。3个月后动物麻醉开胸暴露心脏。测量右房有效不应期。测量窦房结传导时间(SCT)和窦房结恢复时间(SRT)评价窦房结功能。采用心房短阵刺激评价房间传导时间(IACT)和心房对刺激的反应。用放免法测量血浆和心房组织血管紧张素Ⅱ(AngⅡ)浓度。取心房组织制作切片,Masson染色,测量纤维组织占总区域的百分比。结果:实验组IACT和SRT较对照组缩短(P<0.01或P<0.05)。对照组有9例诱发出房颤,实验组有4例诱发出房颤(P<0.05)。实验组左、右房组织匀浆AngⅡ浓度较对照组降低(均P<0.01)。实验组大鼠左房和右房纤维化程度均较对照组明显减轻(均P<0.01)。结论:老龄鼠长期应用依那普利后房颤诱发率降低,窦房结功能改善。Objective：To study the effect of enalapril on inducible atrial fibrillation （AF） in old rats. Methods：Old male Wistar rats were randomly divided into control group （n=12） and experimental group （n=13）. Rats in control group were fed routinely. Rats were fed with enalapril besides normal diet in experimental group for three months. Rats were then anesthetized,thoracotomy was performed and pericardium was opened to expose heart. Right atrium effective refractory period （ERP） was measured. Sinus conduction time （SCT） and sinus recovery time （SRT） were measured for evaluating sinus function. Interatrial conduction time（IACT） and atrium response to burst pacing were evaluated in vivo. Plasma angiotensinⅡ level and atrial tissue angiotensinⅡ level were determined by radioimmunoassay. Sections were cut from the tissue of atrium and stained with Masson trichrome. The ratio of the area occupied by interstitial to the total area was measured. Results：Contrast to control group,IACT and SRT were shorter in experimental group （P〈0.01 and P〈0.05 respectively）. AF were induced in 9 rats in control group and 4 rats in experimental group（P〈0.05）. AngiotensinⅡconcentration was significantly decreased in right and left atrium tissues of experimental group compared with that in control group （P〈0.01）. A significant decrease in interstitial atrial fibrosis was presented in experimental group compared with that of control group （P〈0.01）. Conclusion：Inducible atrial fibrillation rate was decreased in old rats after treatment with enalapril. This effect maybe resulted from the inhibited local atrium reninangiotensin system and improved sinus node function by enalapril.

关 键 词：依那普利 心房颤动 肾素-血管紧张素系统 纤维化 大鼠 WISTAR 

分 类 号：R541.75[医药卫生—心血管疾病] R544.105[医药卫生—内科学]