牛Smad 9基因cDNA克隆及生物信息学分析  

Cloning and Tissues Expression Analysis of cDNA Encoding the Bovine Smad 9 Gene

作　　者：张小辉[1,2] 高雪[1] 任红艳[1] 陈金宝[1] 许尚忠[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094 [2]河南科技大学动物科技学院,河南洛阳471003

出　　处：《云南农业大学学报（自然科学版）》2010年第1期75-79,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：公益性行业(农业)科研专项(nyhyzx07-035-07);"十一五"国家科技支撑计划项目(2007BAD55B02;2006BAD04A16)

摘　　要：Smad 9属于R-Smads,在TGF-β信号传导过程中起着重要作用。本研究利用生物信息学方法,结合RT-PCR技术和SMARTRACE技术,得到全长为1 871 bp的牛Smad 9基因cDNA序列。通过核酸序列分析发现,牛Smad 9基因编码345个氨基酸残基,牛Smad 9蛋白无跨膜区和信号肽序列,该蛋白与人、小鼠、大鼠和红原鸡在氨基酸序列上分别有78.84%,79.21%,77.88%和70.38%的同源性。通过多组织RT-PCR分析发现,牛Smad 9基因的组织表达谱很广,在卵巢、肝、肌肉、小肠、脂肪、子宫、肾脏、心肌、肺、胰腺、睾丸、乳腺组织中均有表达。The Smad 9 is one of the members of R - Smads and played an important role in the signal transmission of TGF - β. The bovine Smad 9 gene cDNA was cloned by RT - PCR, RACE - PCR and got a 1 871 bp full-long cDNA sequence. The bovine Smad 9 gene codes 345 amino acids and shares 78. 84% , 79.21% , 77. 88% and 70. 38% similarity in amino acid sequences with human, mouse, rat and jungle fowl respectively. The bovine Smad 9 had no signal peptide and transmembrane helix. Smad 9 mRNA was found in ovary, liver, skeleton muscle, small intestine, fat, uterus, kidney, heart, lung, pancreas, testis and galactophore by RT -PCR, which indicated Smad 9 was broad expressed in bovine

关 键 词：SMAD 9 RT—PCR 生物信息学 组织表达谱 

分 类 号：S823.2[农业科学—畜牧学]