浙江省桃花水母遗传多样性的RAPD分析  

RAPD Analysis on Genetic Diversity of Craspedacusta in Zhejiang

作　　者：徐善良[1,2] 周剑君[1] 王丹丽[1] 孙雪[2] 

机构地区：[1]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211 [2]宁波大学海洋生物工程浙江省重点实验室,浙江宁波315211

出　　处：《宁波大学学报（理工版）》2010年第1期1-6,共6页Journal of Ningbo University：Natural Science and Engineering Edition

基　　金：教育部长江学者与创新团队项目(IRT0734);浙江省自然科学基金(Y307063)

摘　　要：应用RAPD技术对浙江省杭州、宁波、温州和金华4个地区桃花水母群体的遗传多样性进行了分析，从80个随机引物中筛选出10个引物对4个群体28个个体的基因纽DNA进行扩增，共检测到230个位点，分子片段在200-2000bp之间，其中多态位点124个，占53．91％；群体间最大的遗传距离为0．6506，最小的遗传距离为0．2451，平均遗传距离为0．4825；各群体的多态位点比例俨）为27．78％-76．47％，平均杂合度（H）为0．0998-0．2977，shannon多样性指数（I）为0．1500-0．4390．整个群体的H，I和遗传分化指数（Gst）分别为0．2610、0．3931和0．2249．研究结果表明：桃花水母的遗传多样性较丰富，其中宁波象山和杭州玉泉群体遗传多样性水平低于整体水平，温州平阳和金华永康群体的遗传多样性水平高于整体水平，且群体内存在较大的遗传分化．Samples of Craspedacusta are collected from 4 regions in Zhejiang Province including Ningbo, Hangzhou, Jinhua and Wenzhou, and 28 individuals from 4 groups are analyzed by the selection of 10 random primers from the total 80 random primers. Total of 230 RAPD markers are detected in these individuals, and the size of DNA fragments is from 0.2 kb to 2 kb, 124 of 230 markers detected are polymorphic which account for 53.91%. The genetic distance between stocks is found to range from 0.245 1 to 0.650 6, and the average genetic distance to be 0.482 5. The proportion of polymorphic loci （P） is between 27.78%-76.47%, with the average heterozygosity （/4） being 0.099 8-0.297 7, and the index of Shannon （/） being 0.150 0-0.439 0. The whole group H, 1 and Gst are found to be 0.261 0, 0.393 1 and 0.224 9, respectively. The results suggest that the overall genetic Craspedacusta is significantly diverse and the level of genetic diversity in Ningbo Xiangshan and Hangzhou Yuquan groups is lower than average. In contrast, Wenzhou Pingyang and Jinhua Yongkang groups turn out to be higher in terms of genetic diversity, indicating that there must be obvious genetic variation in stocks.

关 键 词：桃花水母 遗传多样性 RADP 浙江省 

分 类 号：S917.4[农业科学—水产科学]