杨树R2R3 MYB基因PeMYBL1的克隆及表达  

Cloning and Characterization of PeMYBL1,an R2R3 MYB Gene from Poplar

作　　者：宿红艳[1] 王磊[1] 王仲礼[1] 冯培勇[1] 张晔[1] 

机构地区：[1]鲁东大学生命科学学院,烟台264025

出　　处：《林业科学》2010年第1期142-146,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30740017);鲁东大学学科建设项目

摘　　要：An R2R3 MYB gene,PeMYBL1,was isolated from male inflorescence of Populus×euramericana by homologous cloning combined with in silico cloning techniques.The full length of PeMYBL1 cDNA was 1 094 bp encoding 276 amino acids.The deduced amino acid sequence contained two conserved MYB domains near the N-terminus,a conserved E1 motif and an acidic Ser/Thr rich region toward its C terminus.Phylogenetic analysis revealed that PeMYBL1 was clustered with AtMYB85 from Arabidopsis thaliana,ZmMYBL1 from Zea mays,OsMYB15 from Oryza sativa and ODORANT1 from Petunia hybrida.Furthermore,expression analysis by RT-PCR showed that PeMYBL1 was expressed in root,stem,leaf,male and female infloresences,and abundantly accumulated in male inflorescences.The expression level of PeMYBL1 increased with the development of male inflorescences,indicating that PeMYBL1 is closely related to male flower development.An R2R3 MYB gene, PeMYBL1, was isolated from male inflorescence of Populus x euramericana by homologous cloning combined with in silico cloning techniques. The full length of PeMYBL1 cDNA was 1 094 bp encoding 276 amino acids. The deduced amino acid sequence contained two conserved MYB domains near the N-terminus, a conserved E1 motif and an acidic Ser/Thr rich region toward its C terminus. Phylogenetie analysis revealed that PeMYBL1 was clustered with AtMYB85 from Arabidopsis thaliana, ZmMYBL1 from Zea mays, OsMYB15 from Oryza sativa and ODORANT1 from Petunia hybrida. Furthermore, expression analysis by RT-PCR showed that PeMYBL1 was expressed in root, stem, leaf, male and female infloresenees, and abundantly accumulated in male inflorescences. The expression level of PeMYBL1 increased with the development of male inflorescences, indicating that PeMYBL1 is closely related to male flower development.

关 键 词：杨树 MYB基因 克隆 表达分析 雄花序 

分 类 号：S718.46[农业科学—林学] Q943.2[生物学—植物学]