猪屎豆丛枝病植原体的分子检测与鉴定  

Molecular Identification of Crotalaria Witches'-Broom Phytoplasma

作　　者：李永[1] 徐启聪[1] 田国忠[1] 郭民伟[1] 朴春根[1] 

机构地区：[1]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室,北京100091

出　　处：《林业科学》2010年第1期163-168,共6页Scientia Silvae Sinicae

基　　金：国家科技基础条件平台建设项目(2005DKA21207);国家自然科学基金项目(30471393)资助

摘　　要：Crotalaria witches’-broom phytoplasma (CrWB) collected from Hainan Province,was classified and identified by sequencing techniques and Virtual RFLP.Two different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp gene.The PCR-amplified products were cloned and sequenced.The results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches’-broom phytoplasma shared 99.9 % similarity with peanut witches’-broom phytoplasma in 16S rRNA gene and 99.9% in rp gene.Crotalaria witches’-broom phytoplasma is sorted into Peanut witches’-broom group(16SrII),16SrII-A,according to the results of Virtual RFLP of 16S rDNA,phylogenetic analysis of 16S rRNA and rp gene,which is related to Ca.Phytoplasma australasiae.Crotalaria witches＇-broom phytoplasma （CrWB） collected from Hainan Province, was classified and identified by sequencing techniques and Virtual RFLP. Two different type universal primer pairs of phytoplasma were used to amplify a 1.8 kb fragment of 16S rDNA and a 1.3 kb fragment of rp gene. The PCR-amplified products were cloned and sequenced. The results of sequencing and homologuous comparison with other phytoplasmas showed that Crotalaria witches＇ -broom phytoplasma shared 99.9 % similarity with peanut witches＇ -broom phytoplasma in 16S rRNA gene and 99.9% in rp gene. Crotalaria witches ＇-broom phytoplasma is sorted into Peanut witches ＇-broom group （16SrII）, 16SrII-A, according to the results of Virtual RFLP of 16S rDNA, phylogenetic analysis of 16S rRNA and rp gene, which is related to Ca. Phytoplasma australasiae.

关 键 词：猪屎豆丛枝病 植原体 分子鉴定 序列测定 

分 类 号：S432.4[农业科学—植物病理学]